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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant human HO-1 protein
- 亚型:
IgG2a
- 保存条件:
Store at -20°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性:
Monoclonal
- 标记物:
CoraLite® Plus 488 Fluorescent Dye
- 适应物种:
human, mouse, rat, pig
- 保质期:
详见产品包装
- 库存:
充足
- 宿主:
Mouse
- 应用范围:
IF-P, FC (Intra)
- 靶点:
HO-1/HMOX1
- 抗体英文名:
CoraLite® Plus 488-conjugated HO-1/HMOX1 Monoclonal antibody
- 抗体名:
CoraLite® Plus 488-conjugated HO-1/HMOX1 Monoclonal antibody
- 规格:
100ul
经过测试的应用
| Positive IF-P detected in | human liver tissue, mouse spleen tissue |
| Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-66743 targets HO-1/HMOX1 in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.
| 经测试应用 | IF-P, FC (Intra) |
| 文献引用应用 | IF |
| 经测试反应性 | human, mouse, rat, pig |
| 文献引用反应性 | mouse |
| 免疫原 |
CatNo: Ag21296 Product name: Recombinant human HO-1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-288 aa of BC001491 Sequence: MERPQPDSMPQDLSEALKEATKEVHTQAENAEFMRNFQKGQVTRDGFKLVMASLYHIYVALEEEIERNKESPVFAPVYFPEELHRKAALEQDLAFWYGPRWQEVIPYTPAMQHYVKRLHEVGRTEPELLVAHAYTRYLGDLSGGQVLKKIAQKALDLPSSGEGLAFFTFPNIASATKFKQLYRSRMNSLEMTPAVRQRVIEEAKTAFLLNIQLFEELQELLTHDTKDQSPSRAPGLRQRASNKVQDSAPVETPRGKPPLNTRSQAPLLRWVLTLSFLVATVAVGLYAM |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | heme oxygenase (decycling) 1 |
| 别名 | HMOX1, HO-1, Heme oxygenase 1 soluble form, Heme oxygenase 1, heme oxygenase (decycling) 1 |
| 计算分子量 | 33 kDa |
| 观测分子量 | 33 kDa |
| GenBank蛋白编号 | BC001491 |
| 基因名称 | HO-1 |
| Gene ID (NCBI) | 3162 |
| RRID | AB_2883379 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P09601 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Heme oxygenase (HMOX1) catalyzes the first and rate-limiting step in the degradation of heme to yield equimolar quantities of biliverdin Ixa, carbon monoxide (CO), and iron. It has 3 isoforms: HO-1 is highly inducible, whereas HO-2 and HO-3 are constitutively expressed (PMID:10194478). Heme oxygenase-1 (HO-1) is expressed in many tissues and vascular smooth muscle cells, and endothelial cells (PMID:15451051) and has been identified as an important endogenous protective factor induced in many cell types by various stimulants, such as hemolysis, infiammatory cytokines,oxidative stress, heat shock, heavy metals, and endotoxin (PMID: 11522663). And the full-length HO-1 is very unstable and susceptible to truncation that generates an inactive, soluble form (28 kDa) (James R. Reed, Pharmacology, 535-568).
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文献和实验| Species | Application | Title |
|---|---|---|
| mouse | IF | J Mol Cell Cardiol Transcriptional coactivation of NRF2 signaling in cardiac fibroblasts promotes resistance to oxidative stressAuthors - Lisa K McClendon |
:单克隆抗体设立相应亚型的对照,而多克隆抗体需要对应种属的 IgG 作为对照。用血球计数法或是其他方法对细胞计数。 1. 将 0.5-1 x 106 个细胞分到各检测管中(依照体积)。 2. 各管加入 2-3 ml 孵育缓冲液漂洗,离心回收细胞。重复一次。 3. 将每个管中的细胞重悬在 100 µl 孵育缓冲液中。 4. 室温,孵育缓冲液封闭 10 分钟。 5. 按合适的稀释比例在检测管中加入未标记,生物素标记或是荧光素标记的一抗。具体的用量参考抗体各自的说明书。
增殖及计数分析 02 细胞鉴定和免疫分型 荧光标记的CD抗体(Abs)被广泛用于流式细胞术进行细胞识别和免疫分型,至少有15种人类T细胞亚型被描述出来(例如,CD4+,CD8+等)。Incucyte®利用相同的原理,对标记方法进行改进: 将抗CD抗体结合到同型匹配的带有荧光标签的Fc区域靶向Fab片段,便可通过简单的一步混合来完成标记。在检测中使用一种非干扰性的背景荧光抑制染料,以改善488 nm的荧光信号,保护细胞的同时提供更灵活的分析方案。 03 细胞激活和分化 免疫
for plague as a critical percolation phenomenon 血红素加氧酶可促进疟原虫肝期感染 编辑本段 回目录 5月15日出版的《细胞—宿主与微生物》(Cell Host & Microbe)上刊载了一篇有关血红素加氧酶可促进疟原虫肝期感染的研究论文。 临床无症状的疟原虫肝期是形成疟疾感染和疾病的必经阶段。经研究发现血红素加氧酶-1(HO-1,由Hmox1编码)的表达在感染伯氏疟原虫及约氏疟原虫孢子的肝脏中呈上调趋势
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