- ￥2800
- Proteintech
- CL488-66217
- 2025年10月13日
- IF/ICC
- Mouse
- human
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- 文献和实验
- 技术资料
- 免疫原：
Recombinant human ORM2 protein
- 亚型：
IgG1
- 保存条件：
Store at -20°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
CoraLite® Plus 488 Fluorescent Dye
- 适应物种：
human
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Mouse
- 应用范围：
IF/ICC
- 靶点：
ORM2-Specific
- 抗体英文名：
CoraLite® Plus 488-conjugated ORM2-Specific Monoclonal antibody
- 抗体名：
CoraLite® Plus 488-conjugated ORM2-Specific Monoclonal antibody
- 规格：
100ul
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-66217 targets ORM2-Specific in IF/ICC applications and shows reactivity with human samples.
| 经测试应用 | IF/ICC |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag18984 Product name: Recombinant human ORM2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-201 aa of BC015964 Sequence: MALSWVLTVLSLLPLLEAQIPLCANLVPVPITNATLDRITGKWFYIASAFRNEEYNKSVQEIQATFFYFTPNKTEDTIFLREYQTRQNQCFYNSSYLNVQRENGTVSRYEGGREHVAHLLFLRDTKTLMFGSYLDDEKNWGLSFYADKPETTKEQLGEFYEALDCLCIPRSDVMYTDWKKDKCEPLEKQHEKERKQEEGES |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | orosomucoid 2 |
| 别名 | AGP 2, AGP B, AGP B', AGP2, Alpha 1 acid glycoprotein 2, OMD 2, ORM2, orosomucoid 2 |
| 计算分子量 | 24 kDa |
| 观测分子量 | 45 kDa |
| GenBank蛋白编号 | BC015964 |
| 基因名称 | ORM2 |
| Gene ID (NCBI) | 5005 |
| RRID | AB_2919302 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P19652 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ORM2, also named as AGP2 and OMD 2, belongs to the calycin superfamily and Lipocalin family. It functions as transport protein in the blood stream. ORM2 binds various hydrophobic ligands in the interior of its beta-barrel domain. It also binds synthetic drugs and influences their distribution and availability. ORM2 appears to function in modulating the activity of the immune system during the acute-phase reaction. ORM2 is one of the conserved endoplasmic reticulum membrane proteins which regulating lipid homeostasis and protein quality control. This antibody can specially recognize ORM2. It recognizes a band about 45 kDa in human plasma which may be due to the glycosylation of ORM2 or the dimer formation of the protein.
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文献和实验Site‐Specific Protein Labeling with SNAP‐Tags
conjugated species‐specific secondary antibodies (Invitrogen or Li‐COR, http://www.licor.com) NeutrAvidin, DyLight 800 conjugate (Thermo Scientific
on Veeco DI SPMLab software. 5. Photographs The photographs, showing difference in intensity of Alexa488 signal on glass, SIF, and SACS, were taken with Canon 300D® digital SLR camera. The illumination source was Ti: Sapphire laser (Mai Tai
细胞仪检测。或者继续 E1 步 DNA 染色。 E. DNA 染色（可选） 1. 将细胞重悬在 0.5 ml DNA 染色液中（例如 DRAQ5® #4084）。 2. 室温至少孵育 5 分钟。 3. 流式细胞仪进行分析。 ☀ 推荐的二抗： 抗兔： Anti-Rabbit IgG（H+L）， F（ab'）2 Fragment（Alexa Fluor® 488 Conjugate）#4412 Anti-Rabbit IgG（H+L）， F（ab
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