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CoraLite® Plus 488 Anti-Mouse

MHC Class II (I-A/I-E) (M5/114.15.2) CL488-65122
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  • ¥800 - 1990
  • Proteintech已认证
  • CL488-65122
  • 2025年10月13日
  • IF/ICC, FC
  • Rat
  • mouse
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原

      None

    • 亚型

      IgG2b, kappa

    • 保存条件

      Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

    • 克隆性

      Monoclonal

    • 标记物

      CoraLite® Plus 488 Fluorescent Dye

    • 适应物种

      mouse

    • 保质期

      详见产品包装

    • 库存

      充足

    • 宿主

      Rat

    • 应用范围

      IF/ICC, FC

    • 靶点

      MHC Class II (I-A/I-E)

    • 抗体英文名

      CoraLite® Plus 488 Anti-Mouse MHC Class II (I-A/I-E) (M5/114.15.2)

    • 抗体名

      CoraLite® Plus 488 Anti-Mouse MHC Class II (I-A/I-E) (M5/114.15.2)

    • 规格

      25ug/100ug

    规格:25ug产品价格:¥800.0
    规格:100ug产品价格:¥1990.0

    经过测试的应用

    Positive IF/ICC detected inmouse splenocytes
    Positive FC detected inmouse splenocytes
    For other applications, we recommend the unconjugated version of this antibody, 65122-1-Ig

    推荐稀释比

    应用推荐稀释比
    Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
    Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in 100 μl suspension
    This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
    Sample-dependent, Check data in validation data gallery.

    产品信息

    CL488-65122 targets MHC Class II (I-A/I-E) in IF/ICC, FC applications and shows reactivity with mouse samples.

    经测试应用 IF/ICC, FC
    经测试反应性 mouse
    免疫原

    fusion protein

    宿主/亚型 Rat / IgG2b, kappa
    抗体类别 Monoclonal
    产品类型 Antibody
    全称 histocompatibility 2, class II antigen A, alpha
    别名 H2Aa, Ia1, Ia 1, H2-Aa, H 2Aa
    GenBank蛋白编号BC031711
    基因名称 MHC Class II
    Gene ID (NCBI) 14960
    RRIDAB_2883191
    偶联类型 CoraLite® Plus 488 Fluorescent Dye
    最大激发/发射波长493 nm / 522 nm
    形式Liquid
    纯化方式Affinity purification
    UNIPROT IDP14434
    储存缓冲液 PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
    储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

    背景介绍

    Major histocompatibility complex (MHC) class II molecules are heterodimeric transmembrane glycoproteins that are expressed on the surface of antigen-presenting cells such as dendritic cells, macrophages, and B cells. The M5/114.15.2 antibody detects polymorphic determinants present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2b, H-2d, H-2q, H-2p, H-2r and H-2u haplotypes but not from mice carrying the H-2s or H-2f haplotypes (PMID: 6170707). This antibody recognizes both I-A and I-E subregion-encoded Ia glycoproteins (I-Ab, I-Ad, I-Aq, I-Ed, I-Ek, not I-Af, I-Ak, or I-As). M5/114 mAb is reported to inhibit I-A subregion-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not the H-2f, H-2k, H-2s haplotypes (PMID: 6173436).

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    CoraLite® Plus 488 Anti-Mouse MHC Class II (I-A/I-E) (M5/114.15.2) CL488-65122
    ¥800 - 1990