- ￥2800
- Proteintech
- Biotin-60042
- 2025年10月13日
- IHC
- Mouse
- human, mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human CXCR4 protein
- 亚型：
IgM
- 保存条件：
Store at -20°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
Biotin
- 适应物种：
human, mouse
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Mouse
- 应用范围：
IHC
- 靶点：
CXCR4
- 抗体英文名：
Biotin-conjugated CXCR4 Monoclonal antibody
- 抗体名：
Biotin-conjugated CXCR4 Monoclonal antibody
- 规格：
100ul
经过测试的应用
| Positive IHC detected in | human breast cancer tissue, mouse spleen tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
Biotin-60042 targets CXCR4 in IHC applications and shows reactivity with human, mouse samples.
| 经测试应用 | IHC |
| 经测试反应性 | human, mouse |
| 免疫原 |
CatNo: Ag1528 Product name: Recombinant human CXCR4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 192-352 aa of BC020968 Sequence: NDLWVVVFQFQHIMVGLILPGIVILSCYCIIISKLSHSKGHQKRKALKTTVILILAFFACWLPYYIGISIDSFILLEIIKQGCEFENTVHKWISITEALAFFHCCLNPILYAFLGAKFKTSAQHALTSVSRGSSLKILSKGKRGGHSSVSTESESSSFHSS |
| 宿主/亚型 | Mouse / IgM |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | chemokine (C-X-C motif) receptor 4 |
| 别名 | CD184, CXC R4, CXCR 4, CXCR4, CXCR4B, D2S201E, FB22, Fusin, HM89, HSY3RR, LAP3, LCR1, LESTR, SDF 1 receptor, WHIM |
| 计算分子量 | 352 aa, 40 kDa |
| 观测分子量 | 60~70 kDa |
| GenBank蛋白编号 | BC020968 |
| 基因名称 | CXCR4 |
| Gene ID (NCBI) | 7852 |
| RRID | AB_2918944 |
| 偶联类型 | Biotin |
| 形式 | Liquid |
| 纯化方式 | Caprylic acid/ammonium sulfate precipitation |
| UNIPROT ID | P61073 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
C-X-C chemokine receptor type 4 (CXCR4) is a widely expressed G protein-coupled seven-transmembrane receptor. CXCL12/SDF-1 is the biological ligand for CXCR4. The binding of CXCL12 to CXCR4 induces intracellular signaling through several divergent pathways initiating signals related to chemotaxis, cell survival and/or proliferation, increase in intracellular calcium, and gene transcription (PMID: 20484021). CXCR4 also functions as a coreceptor for HIV-1 entry (PMID: 9427609). CXCR4 has a calculated molecular weight of 40 kDa. In addition to the predicted species of 45-47 kDa for glycosylated CXCR4 monomers, due to ubiquitination, dimeration, and different degrees of glycosylation, additional species can also exist and have been reported in some researches, including 67 kDa (PMID: 23917520), 55, 67, 87 kDa (PMID: 20028517), 80 kDa (PMID: 10506573), 47, 50, 62, and 98 kDa (PMID: 16204649).
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文献和实验Biotin-4-fluorescein (B4F) is a convenient molecular probe for (strept)avidin and for unlabeled biotin in homogeneous fluorescence assays. The primary standard is a 16 μM working solution of d-biotin which is used to titrate an aliquot
【交流】DIG/Biotin标记的Southern/Northern杂交专题讨论
Southern印迹杂交（Southern blot）是1975年由英国人southern创建，是研究DNA图谱的基本技术，在遗传病诊断、DNA图谱分析及PCR产物分析等方面有重要价值。 早期的Southern印迹是将凝胶中的DNA变性后，经毛细管的虹吸作用，转移到硝酸纤维膜上。印迹方法如电转法、真空转移法；滤膜发展了尼龙膜、化学活化膜（如APT、ABM纤维素膜）等。利用Southern印迹法可进行克隆基因的酶切、图谱分析、基因组中某一基因的定性及定量分析、基因突变分析及限制性片断长度多态
Conjugation of Antibodies to Biotin
Antibodies can be conjugated to biotin by a number of chemical means. They can then be used in immunochemical procedures in conjunction with secondary reagents coupled to biotin-binding protein proteins such as avidin.
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