- ￥2800
- Proteintech
- Biotin-28076
- 2025年10月13日
- IHC
- Rabbit
- Human, mouse, rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human PD-L1/CD274 protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Biotin
- 适应物种：
Human, mouse, rat
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
IHC
- 靶点：
PD-L1/CD274 (C-terminal)
- 抗体英文名：
Biotin-conjugated PD-L1/CD274 (C-terminal) Polyclonal antibody
- 抗体名：
Biotin-conjugated PD-L1/CD274 (C-terminal) Polyclonal antibody
- 规格：
100ul
经过测试的应用
| Positive IHC detected in | human tonsillitis tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
Biotin-28076 targets PD-L1/CD274 (C-terminal) in IHC applications and shows reactivity with Human, mouse, rat samples.
| 经测试应用 | IHC |
| 经测试反应性 | Human, mouse, rat |
| 文献引用反应性 | mouse |
| 免疫原 |
CatNo: Ag27557 Product name: Recombinant human PD-L1/CD274 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 181-290 aa of BC074984 Sequence: TTNSKREEKLFNVTSTLRINTTTNEIFYCTFRRLDPEENHTAELVIPELPLAHPPNERTHLVILGAILLCLGVALTFIFRLRKGRMMDVKKCGIQDTNSKKQSDTHLEET |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | CD274 molecule |
| 别名 | B7 H, B7 H1, B7 homolog 1, B7H1, CD274, CD274 molecule, PD L1, PDCD1 ligand 1, PDCD1L1, PDCD1LG1, PDL1, Programmed death ligand 1 |
| 计算分子量 | 290 aa, 33 kDa |
| 观测分子量 | 45-50 kDa |
| GenBank蛋白编号 | BC074984 |
| 基因名称 | PD-L1 |
| Gene ID (NCBI) | 29126 |
| RRID | AB_2918943 |
| 偶联类型 | Biotin |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NZQ7 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PD-L1, also known as CD274 or B7H1, stands for programmed cell death ligand 1. It is a type I transmembrane protein that is thought to repress immune responses by binding to its receptor (PD1), thus inhibiting T-cell activation, proliferation, and cytokine production. It contains V-like and C-like immunoglobulin domains. PD-L1 expression is regulated by various cytokines, such as TNF-α or LPS (ISSN: 1848-7718). Increased expression of this protein in certain types of cancers, e.g., renal cell carcinoma or colon cancer, correlates with poor prognosis.
What is the molecular weight of PD-L1?
Depending on the isoform, the calculated molecular weight of the protein varies between 20 and 33 kDa (176-290 aa).
What are the isoforms of PD-L1?
According to NCBI, three different isoforms have been identified. There are significant differences in the untranslated and protein coding regions.
What is the subcellular localization and tissue specificity of PD-L1?
It is predicted to localize in the plasma membrane of various cell types, with a particularly high expression in placental trophoblast and subsets of immune cells. High levels of PD-L1 protein have also been detected in lung and colon tissues.
What is the function of PD-L1 in immune responses?
PD-L1 is critical for the induction and maintenance of immune self-tolerance during infection or inflammation in normal tissues. The interaction of PD-L1 and its receptors is responsible for preventing auto-immune phenotypes and balancing the overall immune response in situations such as pregnancy or tissue allografts. The interaction between PD-L1 and PD-1 or B7.1 starts an inhibitory signaling cascade, which results in the decreased proliferation of antigen-specific T-cells and increased survival of regulatory T-cells (PMID: 15240681).
How can PD-L1's implication in cancer be used as a drug target?
In certain tumors, high expression of PD-L1 serves as a stop-sign to inhibit the recognition of cancer cells by T-cells (PMID: 23087408). The interaction between PD-L1 and its receptors (PD1 and B7.1) is a mechanism for the tumor to evade the host immune response (PMID: 29357948). Several mAbs have been developed to target that interaction and thus prevent the inactivation of cytotoxic T-cells by the tumor (PMIDs: 23890059, 18173375).
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文献和实验| Species | Application | Title |
|---|---|---|
| mouse | ACS Appl Mater Interfaces Harnessing Spatiotemporal-Specific Tumorous Exosome Dynamics: Ultra-Sensitive Lanthanide Luminescence Detection Strategy Enabled by Exosomal Membrane Engineering for Melanoma Immunotherapy MonitoringAuthors - Nanhang Zhu |
Biotin labeling and hybridization of probe to chromosomes
quality) at 70 C for 2 min. Immediately transfer the slides into an ethanol series (70 %, 95 %, and 100 %, 5 min each at 20 C), and air dry the slides. Add 10 µl of the hybridization mixture to each slide and cover with an '18 x 18' mm
Preparation and purification of biotin-labeled probe for ISH
Preparation and purification of biotin-labeled probe for ISH I. Biotin-labeling of plasmid or genomic DNA. Suggested chemicals: Nick Translation Kit, Enzo Diagnostics Cat. No. 42803. Prepare the following
Enzymatic Fluorescence and Biotin Labeling of Primers for PCR Sequencing
, in particular. Using radioactive labeling techniques, it has generally been accepted that 5′-terminal phosphorylation of the primer prior to the sequencing reaction ( 2 ) is the most reliable approach to obtaining sequence information. However, the combination
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