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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
None
- 亚型:
IgG2b, kappa
- 保存条件:
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性:
Monoclonal
- 标记物:
APC Fluorescent Dye
- 适应物种:
human
- 保质期:
详见产品包装
- 库存:
充足
- 宿主:
Mouse
- 应用范围:
IF/ICC, IF-P, FC
- 靶点:
CD45RA
- 抗体英文名:
APC Anti-Human CD45RA (HI100)
- 抗体名:
APC Anti-Human CD45RA (HI100)
- 规格:
25tests/100tests
| 规格: | 25tests | 产品价格: | ¥600.0 |
|---|---|---|---|
| 规格: | 100tests | 产品价格: | ¥1200.0 |
经过测试的应用
| Positive IF-P detected in | human tonsillitis tissue |
| Positive IF/ICC detected in | human peripheral blood mononuclear cells |
| Positive FC detected in | human PBMCs |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:10-1:100 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:150-1:600 |
| Flow Cytometry (FC) | FC : 5 ul per 10^6 cells in 100 μl suspension |
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
APC-65108 targets CD45RA in IF/ICC, IF-P, FC applications and shows reactivity with human samples.
| 经测试应用 | IF/ICC, IF-P, FC |
| 经测试反应性 | human |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Mouse / IgG2b, kappa |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | protein tyrosine phosphatase, receptor type, C |
| 别名 | CD45, EC:3.1.3.48, GP180, HI100, L CA |
| GenBank蛋白编号 | BC014239 |
| 基因名称 | CD45 |
| Gene ID (NCBI) | 5788 |
| ENSEMBL Gene ID | ENSG00000081237 |
| RRID | AB_2882997 |
| 偶联类型 | APC Fluorescent Dye |
| 最大激发/发射波长 | 650 nm / 660 nm |
| 形式 | Liquid |
| 纯化方式 | Affinity purification |
| UNIPROT ID | P08575 |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45 exists as multiple isoforms due to alternative splicing of three exons (4, 5, and 6, designated A, B, and C) in the extracellular domain (PMID: 12414720). CD45RA is expressed on naïve T cells, B cells, and monocytes (PMID: 1830500; 14687231).
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