- ￥1500 - 3280
- Proteintech
- 80488-1-RR
- 2025年10月13日
- WB, IHC, IF/ICC, ELISA, ChIP-qPCR
- Rabbit
- human, mouse, rat, pig, zebrafish
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
None
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Recombinant
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat, pig, zebrafish
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IF/ICC, ELISA, ChIP-qPCR
- 靶点：
Beta Catenin
- 抗体英文名：
Beta Catenin Recombinant monoclonal antibody
- 抗体名：
Beta Catenin Recombinant monoclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1500.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2480.0 |
| 规格： | 150ul | 产品价格： | ￥3280.0 |
经过测试的应用
| Positive WB detected in | A431 cells, zebrafish tissue, HeLa cells, HEK-293 cells, MCF-7 cells, LNCaP cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells, pig brain tissue |
| Positive IHC detected in | human liver cancer tissue, human breast cancer tissue, human colon cancer tissue, human rectal cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
| Positive ChIP-qPCR detected in | HCT 116 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:1000-1:4000 |
| CHIP-QPCR | CHIP-QPCR : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
80488-1-RR targets Beta Catenin in WB, IHC, IF/ICC, CoIP, ELISA, ChIP-qPCR applications and shows reactivity with human, mouse, rat, pig, zebrafish samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA, ChIP-qPCR |
| 文献引用应用 | WB, IF, CoIP |
| 经测试反应性 | human, mouse, rat, pig, zebrafish |
| 文献引用反应性 | human |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | catenin (cadherin-associated protein), beta 1, 88kDa |
| 别名 | 2J24, b cat, B-catenin, beta-Catenin, Catenin beta 1 |
| 计算分子量 | 781 aa, 86 kDa |
| 观测分子量 | 92 kDa |
| GenBank蛋白编号 | BC058926 |
| 基因名称 | Beta Catenin |
| Gene ID (NCBI) | 1499 |
| ENSEMBL Gene ID | ENSG00000168036 |
| RRID | AB_2918895 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P35222 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin is a 92-kDa protein, originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222)
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文献和实验| Species | Application | Title |
|---|---|---|
| human | WB,IF,CoIP | Cell Biol Toxicol M6A modification-mediated LIMA1 promotes the progression of hepatocellular carcinoma through the wnt-βcatenin/Hippo pathwayAuthors - Chao Zhang |
| human | WB | Discov Oncol miR-124-3p inhibits CRC proliferation, migration, and invasion by targeting ITGB1Authors - Jing Li |
liqiuling 怎么才能获取beta catenin的基因？ chuckdouble pubmed里面有Nucleotide.到那里找. liqiuling 不太会看，而且合成的话，很贵，想知道如果想获得别人的惠赠，该怎样做？哪位大侠支个招？不胜感激！ lmnsmu 在NCBI的核酸库中找到其序列，然后设计引物，通过RT-PCR扩增获得该基因
familar 小妹困惑，在此请教大师！ 做了几次beta-catenin的表达（western），都没做出来，目的蛋白的分子量是92，可是，做出来的印记总是在下方，明显的不是目的蛋白的位置。我师兄说，做beta-catenin要提核蛋白才能做出来，可我之前也有做出来过的！请教各位有经验的同志，这样的现象是何解？ woxingwosu 不一定要提核蛋白才能出来。b-catenin在核内核外都有分布。应该
【求助】细胞里的beta catenin 好检测吗？？ 用 western blot
antonio2001 细胞里的beta catenin 好检测吗？？ 用 western blot 和免疫细胞化学 ？？ 2008851029 细胞里的beta-actin非常好检测（我用的是ST的抗体） littlebee83325 什么细胞呢？胞浆蛋白么？ magichunter beta catenin 好检测，细胞全蛋白裂解液上样20uL（15
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