- ￥1500 - 3280
- Proteintech
- 80218-1-RR
- 2025年10月13日
- WB, ELISA, FC (Intra)
- Rabbit
- Human, mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
None
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Recombinant
- 标记物：
Unconjugated
- 适应物种：
Human, mouse
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, ELISA, FC (Intra)
- 靶点：
Phospho-ULK1 (Ser556)
- 抗体英文名：
Phospho-ULK1 (Ser556) Recombinant monoclonal antibody
- 抗体名：
Phospho-ULK1 (Ser556) Recombinant monoclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1500.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2480.0 |
| 规格： | 150ul | 产品价格： | ￥3280.0 |
经过测试的应用
| Positive WB detected in | PC-3 cells, HeLa cells, Calyculin A treated HeLa cells, Calyculin A treated PC-3 cells |
| Positive FC (Intra) detected in | Calyculin A treated PC-3 cells |
This antibody is equivalent to Ser555 in mice.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
80218-1-RR targets Phospho-ULK1 (Ser556) in WB, IHC, IF, FC (Intra), ELISA applications and shows reactivity with Human, mouse samples.
| 经测试应用 | WB, ELISA, FC (Intra) |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | Human, mouse |
| 文献引用反应性 | human, mouse, rat, goat |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | unc-51-like kinase 1 (C. elegans) |
| 别名 | ULK1, KIAA0722, hATG1, EC:2.7.11.1, Autophagy-related protein 1 homolog |
| 观测分子量 | 140 kDa |
| GenBank蛋白编号 | NM_003565 |
| 基因名称 | ULK1 |
| Gene ID (NCBI) | 8408 |
| RRID | AB_2918877 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | O75385 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Unc-51-like-kinase 1 (ULK1) is a target of both the mechanistic target of rapamycin (mTOR) and AMP activated protein kinase (AMPK), whose role is to facilitate the initiation of autophagy in response to starvation. ULK1 is phosphorylated on serine 638 and 758 sites by mTOR in nutrient-rich conditions, inhibiting ULK1 activation by disrupting its binding to AMPK. Upon glucose starvation, dissociation of mTOR from ULK1 and phosphorylation by AMPK leads to the activation of ULK1 activity. The S556 site of ULK1 is one of the major AMPK-dependent phosphorylation sites. (PMID: 30517873, PMID: 21258367)
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文献和实验| Species | Application | Title |
|---|---|---|
| human | WB | J Hazard Mater Graphene oxide disrupted mitochondrial homeostasis through inducing intracellular redox deviation and autophagy-lysosomal network dysfunction in SH-SY5Y cells.Authors - Feng Xiaoli |
| mouse | WB | JCI Insight ZNFX1 promotes AMPK-mediated autophagy against Mycobacterium tuberculosis by stabilizing mRNAAuthors - Honglin Liu |
| human | WB | Sci Rep EYA3 promotes the tumorigenesis of gastric cancer through activation of the mTORC1 signaling pathway and inhibition of autophagyAuthors - Zhen Xu |
| human | WB | Int J Gen Med Sigma-1 Receptor Rescues Autophagy Through AMPK/mTOR Signaling Pathway in Sepsis-Induced Acute Kidney InjuryAuthors - Wei Jiang |
| human | WB | Bioengineered Shikonin induces apoptosis and autophagy via downregulation of pyrroline-5-carboxylate reductase1 in hepatocellular carcinoma cells.Authors - Junli Zhang |
| human | WB | Oxid Med Cell Longev tRNA-Derived Fragment tRF-5009A Regulates Autophagy and Degeneration of Cartilage in Osteoarthritis via Targeting mTORAuthors - Zengfa Deng |
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
the phosphorylation site; therefore, substrate?directed, phosphorylation?state?sensitive, motif?specific (?phospho?motif?) antibodies represent powerful tools to identify novel kinase substrates and to investigate mechanisms of substrate phosphorylation
), and causes activation of KOR. Agonist-activated KOR becomes a substrate for G protein receptor kinase (GRK), which phosphorylates the Ser369 residue at the C-terminal tail of the receptor in the first step in the β-Arrestin-dependent desensitization cascade
XBB.1.5 毒株的传播力为何这么强？北大曹云龙团队最新研究揭示相关机制
导读 SARS-CoV-2 变体 BQ.1.1 和 XBB.1 已在全球范围内传播，前期的研究数据表明，这两个变体与大多数 Omicron 突变体相比具有更优越的生长优势。但是，根据最新的消息，另一 SARS-CoV-2 重组亚型 XBB.1.5 在美国感染人数快速增长，已迅速成为美国的优势毒株，预计很快将会超过现有的流行亚型，并且极有可能引发下一波全球 COVID-19 传播。 图片来源：bioRxiv 尽管 XBB.1.5 拥有着高传播性，但是其内在的分子机制目前仍不清楚。2023 年
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