- ￥1350 - 3000
- Proteintech
- 67794-1-Ig
- 2025年10月13日
- WB, ELISA
- Mouse
- virus
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant virus spike protein protein
- 亚型：
IgG1
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
virus
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Mouse
- 应用范围：
WB, ELISA
- 靶点：
SARS-CoV-2 S protein (944-1214 aa)
- 抗体英文名：
SARS-CoV-2 S protein (944-1214 aa) Monoclonal antibody
- 抗体名：
SARS-CoV-2 S protein (944-1214 aa) Monoclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | ag30682 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67794-1-Ig targets SARS-CoV-2 S protein (944-1214 aa) in WB, ELISA applications and shows reactivity with virus samples.
| 经测试应用 | WB, ELISA |
| 经测试反应性 | virus |
| 免疫原 |
CatNo: Ag30682 Product name: Recombinant virus spike protein protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 944-1214 aa of NC_045512 Sequence: ALGKLQDVVN QNAQALNTLV KQLSSNFGAI SSVLNDILSR LDKVEAEVQI DRLITGRLQS LQTYVTQQLI RAAEIRASAN LAATKMSECV LGQSKRVDFC GKGYHLMSFP QSAPHGVVFL HVTYVPAQEK NFTTAPAICH DGKAHFPREG VFVSNGTHWF VTQRNFYEPQ IITTDNTFVS GNCDVVIGIV NNTVYDPLQP ELDSFKEELD KYFKNHTSPD VDLGDISGIN ASVVNIQKEI DRLNEVAKNL NESLIDLQEL GKYEQYIKWP W |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | SARS-CoV-2 Spike Protein |
| 别名 | COVID-19 RBD, RBD, Spike, spike protein, S protein |
| 计算分子量 | 141 kDa |
| GenBank蛋白编号 | NC_045512 |
| 基因名称 | SARS-COV-2 |
| Gene ID (NCBI) | 43740568 |
| RRID | AB_2918558 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Coronaviruses (CoVs) infect human and animals and cause varieties of diseases, including respiratory, enteric, renal, and neurological diseases. CoV uses its spike protein to recognize ACE2 as its receptors and mediate membrane fusion and virus entry into host cells(PMID: 32221306). Each monomer of trimeric S protein is about 180 kDa, and contains two subunits, S1 and S2,S1 recognizes and binds to host receptors, and subsequent conformational changes in S2 facilitate fusion between the viral envelope and the host cell membrane(PMID: 19198616). Although the amino acid sequences of the S-glycoprotein were found to be different between the various HCoV, the structures showed high similarity, but the best 3D structural overlap shared by SARS-CoV and SARS-CoV-2, consistent with the shared ACE2 predicted receptor (PMID: 32522207). The spike protein of CoVs can be a target for vaccine and therapeutic development (PMID: 19198616).
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文献和实验抗体的检测外，还应该用与其抗原成分相关的其它抗原进行交叉试验，方法可用ELISA、IFA法。例如：①制备抗黑色素瘤细胞的McAb，除用黑色素瘤细胞反应外，还应该用其它脏器的肿瘤细胞和正常细胞进行交叉反应，以便挑选肿瘤特异性或肿瘤相关抗原的单克隆抗体。②制备抗重组的细胞因子的单克隆抗体，应首先考虑是否与表达菌株的蛋白有交叉反应，其次是与其它细胞因子间有无交叉。 2．McAb的Ig类与亚类的鉴定 一般在用酶标或荧光素标记的第二抗体进行筛选时已经基本上确定了抗体的Ig类型
Protein Purification and Analysis
相关专题 Levels of Structure in Protein Primary: A description of all covalent bonds. The sequence of AA residues Secondary: particularly stable arrangements of AA giving rise to recurring structural patterns.
Cell Death Differ：中大五院黄曦团队发现新冠病毒引起肺损伤的分子机制
病理发生。然而，SARS-CoV-2 感染引起肺细胞凋亡的机制仍不清楚。 2022 年 1 月 13 日，中山大学附属第五医院黄曦课题组在 Cell death & differentiation 上发表了题为 SARS-CoV-2 membrane protein causes the mitochondrial apoptosis and pulmonary edema via targeting BOK 研究性论文。该研究证实了病毒膜（M）蛋白会通过靶向 BOK 分子，介导肺
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