- ￥1350 - 3000
- Proteintech
- 25712-1-AP
- 2025年10月13日
- WB, IHC, IF/ICC, IP, ELISA
- Rabbit
- human, rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human KIAA1429 protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, rat
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IF/ICC, IP, ELISA
- 靶点：
VIRMA/KIAA1429
- 抗体英文名：
VIRMA/KIAA1429 Polyclonal antibody
- 抗体名：
VIRMA/KIAA1429 Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | PC-3 cells, SKOV-3 cells, MDA-MB-231 cells |
| Positive IP detected in | MCF-7 cells |
| Positive IHC detected in | human breast cancer tissue, human liver cancer tissue, rat testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
25712-1-AP targets VIRMA/KIAA1429 in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA |
| 文献引用应用 | WB, IHC, IF, IP, CoIP, RIP |
| 经测试反应性 | human, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag22449 Product name: Recombinant human KIAA1429 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-350 aa of BC113380 Sequence: MAVDSAMELLFLDTFKHPSAEQSSHIDVVRFPCVVYINEVRVIPPGVRAHSSLPDNRAYGETSPHTFQLDLFFNNVSKPSAPVFDRLGSLEYDENTSIIFRPNSKVNTDGLVLRGWYNCLTLAIYGSVDRVISHDRDSPPPPPPPPPPPQPQPSLKRNPKHADGEKEDQFNGSPPRPQPRGPRTPPGPPPPDDDEDDPVPLPVSGDKEEDAPHREDYFEPISPDRNSVPQEGQYSDEGEVEEEQQEEGEEDEDDVDVEEEEDEDEDDRRTVDSIPEEEEEDEEEEGEEDEEGEGDDGYEQISSDEDGIADLERETFKYPNFDVEYTAEDLASVPPMTYDPYDRELVPLLY |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | KIAA1429 |
| 别名 | KIAA1429, VIRMA, MSTP054, virilizer |
| 计算分子量 | 1812 aa, 202 kDa |
| 观测分子量 | 200-210 kDa |
| GenBank蛋白编号 | BC113380 |
| 基因名称 | KIAA1429 |
| Gene ID (NCBI) | 25962 |
| RRID | AB_2880204 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q69YN4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
VIRMA (KIAA1429) is a key component of m6A methyltransferase (writer) complex that include METTL3, METTL14, WTAP, VIRMA, HAKAI, ZC3H13, and RBM15. VIRMA mediates methylation in the 3'UTR and around the stop codon, thus affecting alternative polyadenylation. VIRMA has been reported to act as an oncogenic factor in breast cancer and liver cancer. KIAA1429 exhibits differential nuclear and cytoplasmic distribution in colorectal cancer samples, and high cytoplasmic expression of KIAA1429 is associated with poor response to chemotherapy. BRAF-mediated phosphorylation of KIAA1429 facilitates its aggregation in the cytoplasm. Furthermore, cytoplasmic KIAA1429 promotes WNT pathway activation by binding and stabilizing FZD7, thereby further enhancing cancer stemness and oxaliplatin resistance (PMID: 40611274).
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文献和实验| Species | Application | Title |
|---|---|---|
| human | WB | Dev Cell PDGF signaling inhibits mitophagy in glioblastoma stem cells through N6-methyladenosine.Authors - Deguan Lv |
| mouse,human | WB,IP,IHC | Cell Mol Biol Lett KIAA1429-mediated m6A modification of CHST11 promotes progression of diffuse large B-cell lymphoma by regulating Hippo-YAP pathwayAuthors - Xiaomin Chen
|
| human | IF | Cell Rep Pathological α-synuclein dysregulates epitranscriptomic writer METTL3 to drive neuroinflammation in microgliaAuthors - Cameron Miller |
| human | WB | Aging (Albany NY) WTAP promotes myocardial ischemia/reperfusion injury by increasing endoplasmic reticulum stress via regulating m6A modification of ATF4 mRNA.Authors - Jiayi Wang |
| human | WB | Front Oncol m6A Methyltransferase METTL14-Mediated Upregulation of Cytidine Deaminase Promoting Gemcitabine Resistance in Pancreatic Cancer.Authors - Congjun Zhang |
| human | WB,CoIP | Sci Rep The m6A-methylase complex recruits TREX and regulates mRNA export.Authors - Simon Lesbirel
|
因此，综合来说，EarthOx的GST标签抗体 （货号#E022040）是一款同时具有高特异性、高灵敏度、多应用类型以及性价比的GST标签抗体。同时，我们也提供其他品牌的GST标签抗体，如Proteintech的GST多克隆抗体 ――GST PolyClonal Antibody（货号#10000-0-AP）以及Abcam、Santa等多种GST标签抗体产品。更多GST标签抗体以及其他如His、HA、Myc、GFP、Flag等其他标签抗体，请电话垂询。美国EarthOx品牌是一家提供高性价比免疫学和细胞
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