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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant human CCL5 protein
- 亚型:
IgG
- 保存条件:
Store at -20°C. Stable for one year after shipment.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
human
- 保质期:
详见产品包装
- 库存:
充足
- 宿主:
Rabbit
- 应用范围:
WB, ELISA
- 靶点:
RANTES
- 抗体英文名:
RANTES Polyclonal antibody
- 抗体名:
RANTES Polyclonal antibody
- 规格:
50ul/100ul/150ul
| 规格: | 50ul | 产品价格: | ¥1350.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥2200.0 |
| 规格: | 150ul | 产品价格: | ¥3000.0 |
经过测试的应用
| Positive WB detected in | human peripheral blood platelets |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12000-1-AP targets RANTES in WB, IHC, IF, Neutralization, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA |
| 文献引用应用 | WB, IHC, IF, Neutralization |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag2617 Product name: Recombinant human CCL5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-91 aa of BC008600 Sequence: MKVSAAALAVILIATALCAPASASPYSSDTTPCCFAYIARPLPRAHIKEYFYTSGKCSNPAVVFVTRKNRQVCANPEKKWVREYINSLEMS |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chemokine (C-C motif) ligand 5 |
| 别名 | C C motif chemokine 5, CCL5, chemokine (C C motif) ligand 5, D17S136E, EoCP, RANTES, SCYA5, SIS delta, SISd, Small inducible cytokine A5, T cell specific protein P228, T cell specific protein RANTES, TCP228 |
| 计算分子量 | 91 aa, 10 kDa |
| 观测分子量 | 8 kDa |
| GenBank蛋白编号 | BC008600 |
| 基因名称 | CCL5/RANTES |
| Gene ID (NCBI) | 6352 |
| RRID | AB_2877815 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P13501 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RANTES (CCL5) belongs to the CC chemokine family and induces leukocyte migration by binding to specific receptors in the G protein-coupled receptor family. RANTES (CCL5) has been shown in vitro to mediate eosinophil, lymphocyte, neutrophil, and monocyte chemotaxis, and it initiates several other proinflammatory events, such as integrin activation, lipid mediator biosynthesis, and degranulation.
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文献和实验| Species | Application | Title |
|---|---|---|
| mouse | WB | Brain Behav Immun Egln3 expression in microglia enhances the neuroinflammatory responses in Alzheimer's diseaseAuthors - Jiaxin Guan |
| rat | WB | Phytomedicine Integration of transcriptomics and metabolomics reveals the mechanism of Glycyrrhizae Radix Et Rhizoma extract inhibiting CCL5 in the treatment of acute pharyngitisAuthors - Xiaoxiao Han |
| mouse | IHC | Immunology NME4 suppresses NFκB2-CCL5 axis, restricting CD8+ T cell tumour infiltration in oesophageal squamous cell carcinomaAuthors - Shutao Zheng |
iScience Cancer-associated fibroblasts promote enzalutamide resistance and PD-L1 expression in prostate cancer through CCL5-CCR5 paracrine axisAuthors - Zhi Xiong | ||
| mouse | WB,IF | Stem Cells Targeted deletion of Rictor in BMSCs reduces the biological activity of K7M2 cells and mitigates OS-induced bone destructionAuthors - Jinhong Lu |
| human | Neutralization | Ann Transl Med Upregulation of STAT1-CCL5 axis is a biomarker of colon cancer and promotes the proliferation of colon cancer cells.Authors - Mengke Niu |
实验,请根据说明书列出已验证的种属来选择适合你实验的抗体。 (4)样本蛋白的结构性质。了解样本蛋白的结构性质有助于选择最合适的抗体,待测样本蛋白的结构域和样本在提取和处理过程中是否会变性,蛋白空间构象的改变,会影响抗体的免疫亲和反应。 (5)单多克隆抗体的选择。市面上的抗体还是以多克隆抗体为主,一般单克隆抗体特异性强,但亲和力相对小,检测抗原灵敏度相对就低;而多克隆抗体特异性稍弱,但抗体的亲和力强,灵敏度高,但易出现非特异性染色(可以通过封闭等避免
10X TBS:24.2 g Trisbase, 80 g NaCl;用1N HCl调pH为 7.6 7. 甲醇 8. TBS/T缓冲液:1X TBS, 0.1% Tween-20 9. 封闭缓冲液(TBS/T):1X TBS,0.1% Tween-20加5% w/v脱脂奶粉或BSA 10. 一抗的稀释:1X TBS,0.1% Tween-20 加 5% BSA ( 多抗) 或 5%脱脂奶粉( 单抗)。Note: 一般来说,BSA被推荐用于多克隆抗体
DXY网友qingraner问:本人核浆分离做了最少两个月了吧,似乎没有什么进展,我想请问高人指点一下。我很想知道我的配方有什么问题,或者是我的操作有什么需要注意的地方。十分感谢!还有,我检测的方法是用Ikb-α(标细胞质)和Oct-1(标细胞核),似乎抗体也不是很好用。我做的是BT474细胞,breast cancer cell。我的方法如下:1、用Buffer A 洗涤2、500G离心5分钟3、用BufferA+B(2:1)裂解细胞,轻轻混匀,孵育5-10分钟4、12000G离心10分钟
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