- ￥1200 - 7200
- Proteintech
- HRP-66008
- 2025年10月13日
- WB
- Mouse
- recombinant protein
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
None
- 亚型：
IgG2b
- 保存条件：
Store at -20°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
HRP
- 适应物种：
recombinant protein
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Mouse
- 应用范围：
WB
- 靶点：
DYKDDDDK Tag
- 抗体英文名：
HRP-conjugated DYKDDDDK Tag Monoclonal antibody (Binds to FLAG® tag epitope)
- 抗体名：
HRP-conjugated DYKDDDDK Tag Monoclonal antibody (Binds to FLAG® tag epitope)
- 规格：
100ul/500ul/1ml
| 规格： | 100ul | 产品价格： | ￥1200.0 |
|---|---|---|---|
| 规格： | 500ul | 产品价格： | ￥4800.0 |
| 规格： | 1ml | 产品价格： | ￥7200.0 |
经过测试的应用
| Positive WB detected in | Recombinant protein |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:10000-1:100000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
HRP-66008 targets DYKDDDDK Tag in WB, CoIP, ELISA applications and shows reactivity with recombinant protein samples.
| 经测试应用 | WB |
| 文献引用应用 | WB, CoIP, ELISA |
| 经测试反应性 | recombinant protein |
| 文献引用反应性 | mouse |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | DYKDDDDK Tag |
| 别名 | DDDDK tag, DYKDDDDK, DYKDDDDK Tag, HRP conjugated DDDDK tag, HRP-66008, HRP-DDDDK tag |
| 基因名称 | |
| Gene ID (NCBI) | |
| RRID | AB_2883835 |
| 偶联类型 | HRP |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | FLAGTAG |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DYKDDDDK tag, also known as DDK tag, is a hydrophilic tag for recombinant protein technology with the sequence DYKDYKDDDDK. The structure of DYKDDDDK tag has been optimized for compatibility with the proteins it is attached to and unlikely to denature or inactivated them. For its high hydrophilic nature, the DYKDDDDK tag is likely to be located on the surface of a fusion protein, which enables the tag more likely to be accessible to antibodies. This product is generated against 1xDYKDDDDK tag (DYKDDDDK) and can recognize protein containing one or more DYKDDDDK tags, no matter it is/they are located at N-terminal, C-terminal or at internal regions. The binding of this product with DYKDDDDK tagged fusion protein does not depend on calcium ion. The clone of this monoclonal antibody hybridoma is 1E7B4, which is different from any other vendors. This antibody is HRP conjugated DYKDDDDK Tag antibody. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验| Species | Application | Title |
|---|---|---|
| WB | Mol Cancer Exosomal circ_0006896 promotes AML progression via interaction with HDAC1 and restriction of antitumor immunityAuthors - Can Can | |
Ann Rheum Dis Lupus and inflammatory bowel disease share a common set of microbiome features distinct from other autoimmune disordersAuthors - Hao Zhou | ||
| WB | Nat Commun A lung-selective delivery of mRNA encoding broadly neutralizing antibody against SARS-CoV-2 infectionAuthors - Wanbo Tai | |
| WB | Nat Commun FAM222A encodes a protein which accumulates in plaques in Alzheimer's disease.Authors - Tingxiang Yan | |
PLoS Pathog Vibrio splendidus infection promotes circRNA-FGL1-regulated coelomocyte apoptosis via competitive binding to Myc with the deubiquitinase OTUB1 in Apostichopus japonicusAuthors - Ming Guo | ||
| WB | Int Immunopharmacol ZAP facilitates NLRP3 inflammasome activation via promoting the oligomerization of NLRP3Authors - Danhui Qin |
凝集素，四聚体球蛋白，含有糖基结合位点，因此 ConA 是一种糖类结合蛋白。ConA 磁珠能与细胞膜上的糖蛋白结合，从而吸附细胞，使对细胞的实验操作可视化，减少在后续实验过程中的细胞的损失。 不建议使用离心法代替 ConA beads，因为离心法不适合于低细胞数，在洗涤的过程中，损失很大，而且大肠杆菌残留的 DNA 无法进行可靠的校准。 5.做 ChIP-seq 的时候不需要二抗，CUT&Tag 二抗的作用是什么？二抗是必须的吗？可以用带修饰（比如 HRP 修饰）的二抗
Anti-DYKDDDDK tag (L5) Affinity Gel
1. Purifying DYKDDDDK-tag fusion proteins from crude E.coli extracts. 1) Grow the bacteria cells under the condition that induce production of DYKDDDDK-tag fusion proteins. 2) Harvest the cells by centrifugation at 10,000 rpm for 20
大阪大学蛋白质研究所藤井勇树、高木淳一东北大学大学院医学系研究科金子美华、加藤幸成 前言在分析蛋白质的功能时最重要的是如何得到高纯度的蛋白。现今提纯蛋白最常用的方法是亲和标签系统。被统称为「Epitope Tag」的肽标签以及其单克隆抗体组成的系统有FLAG、HA、Myc等多种标签。但是，每个标签系统都有优缺点，并不存在完美的标签系统。于是，在克服了众多难关后我们终于开发出有超高亲和性的新型亲和标签系统“PA Tag System”1）。 PA tag的起源Epitope Tag
技术资料暂无技术资料 索取技术资料
