- ￥1200
- Proteintech
- CL488-66171
- 2025年10月13日
- IF-P
- Mouse
- human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human TF protein
- 亚型：
IgG1
- 保存条件：
Store at -20°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
CoraLite® Plus 488 Fluorescent Dye
- 适应物种：
human
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Mouse
- 应用范围：
IF-P
- 靶点：
Transferrin/TF
- 抗体英文名：
CoraLite® Plus 488-conjugated Transferrin/TF Monoclonal antibody
- 抗体名：
CoraLite® Plus 488-conjugated Transferrin/TF Monoclonal antibody
- 规格：
100ul
经过测试的应用
| Positive IF-P detected in | human liver cancer tissue, human placenta tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-66171 targets Transferrin/TF in IF-P applications and shows reactivity with human samples.
| 经测试应用 | IF-P |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag11668 Product name: Recombinant human TF protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 347-698 aa of BC059367 Sequence: EGTCQEAPTDECKPVKWCALSHHERLKCDEWSVNSVGKIECVSAETTEDCIAKIMNGEADAMSLDGGFVYIAGKCGLVPVLAENYNKSDNCEDTPEAGYFAVAVVKKSASDLTWDNLKGKKSCHTAVGRTAGWNIPMGLLYNKINHCRFDEFFSEGCAPGSKKDSSLCKLCMGSGLNLCEPNNKEGYYGYTGAFRCLVEKGDVAFVKHQTVPQNTGGKNPDPWAKNLNEKDYELLCLDGTRKPVEEYANCHLARAPNHAVVTRKDKEACVHKILRQQQHLFGSNVTDCSGNFCLFRSETKDLLFRDDTVCLAKLHDRNTYEKYLGEEYVKAVGNLRKCSTSSLLEACTFRRP |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | transferrin |
| 别名 | TF, transferrin, Serotransferrin, PRO2086, PRO1557 |
| 计算分子量 | 698 aa, 77 kDa |
| 观测分子量 | 77 kDa |
| GenBank蛋白编号 | BC059367 |
| 基因名称 | TF |
| Gene ID (NCBI) | 7018 |
| RRID | AB_2919293 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P02787 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
What is the tissue specificity of TF?
Transferrin is synthesized mainly in the liver.
What is the calculated molecular weight of TF?
77 kDa
How many iron atoms can bind to TF?
Each of the two lobes of TF has one high-affinity Fe(III) binding site. Since binding of iron and bicarbonate
releases free H+ ions, affinity decreases in acidic conditions, in which TF also remains bound to its receptor.
What is the primary role of TF?
TF serves as a serum metal-binding protein, playing a key role in iron metabolism and delivery for erythropoiesis.
What is the role of TF in human diseases?
TF deficiency results in an exhibition of congenital atransferrinemia (hypotransferrinemia) in which there is a
decrease in hepcidin expression, causing increased iron levels.
Is TF essential for iron delivery?
Congenital atransferrinemia results in an iron overload in some tissues, such as the liver, suggesting that while TF is not essential for iron delivery to all organs, it is needed for iron level regulation.
In what circumstances do TF levels decrease?
Inflammation and iron overload both can cause a decline in TF levels.
How does TF affect hormonal balance?
TF positively regulates expression of hepcidin, a peptide hormone synthesized by the liver.
How does TF deficiency cause increased iron levels?
TF regulates hepcidin, which in turn is a negative regulator of intestinal iron absorption, resulting in TF
deficiency causing both anemia, and, paradoxically, increased iron levels in some tissues.
PMIDs: 23046645, 9635730, 22294463, 24589273
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光色素与免疫球蛋白分子的比值）应该相同为最佳，这对准确设定阴性与阳性细胞的界标有重要意义，切忌使用与MoAb不相匹配的同型对照，最好为同一实验室、采用相同工艺或方法制备（如同一品牌）的产品。 3、流式细胞技术测定细胞内游离钙浓度为何要使用氯化钙.在文献及其他专业网站中都有测定游离钙的方法，具体如下： （1） 钙荧光探针负载； （2）随机测定每管细胞悬液样品（以下简称样品）的单个细胞的荧光强度，记为F值。 （3）加入10%Triton X 100，（破膜用）；加入1 mmol/L氯
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