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文献和实验Free-Hand Bisection of Mouse Oocytes and Embryos
functions and by Western blot and genetic analysis (e.g., RT-PCR). Here we describe a detailed protocol for the free-hand bisection of mouse zona pellucida -free oocytes and embryos on an agar layer using a glass needle.
Cell-Free Protein Synthesis as a Promising Expression System for Recombinant Proteins
Cell-free protein synthesis (CFPS) has major advantages over traditional cell-based methods in the capability of high-throughput protein synthesis and special protein production. During recent decades, CFPS has become an alternative protein
on the use of carrier chromatin, that allows detailed and reproducible epigenetic analysis of as few as 100 cells. The procedure has been validated with primary mouse embryo material, but should be applicable to cells from various sources, including tissue
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