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文献和实验(RSV) attachment glycoprotein (G), which involve the use of lectins and anti-carbohydrate antibodies. Given the role of the G glycoprotein in RSV antigenicity, we also describe procedures based on Western blotting to determine the effect of G protein
Lentiviral Vectors Preparation and Use
as a gene transfer vector. This limitation has been overcome by pseudotyping the viral envelope with vesicular stomatitis virus G (VSV-G) glycoprotein (4 ,5 ). The aim of this chapter is to describe the general procedures developed for the preparation
Preparation and Quantification of Pseudotyped Retroviral Vector
) and cellular receptors, which limit the susceptibility of target cells to retroviral vectors. Murine leukemia retrovirus (MuLV) pseudotyped with vesicular stomatitis virus G glycoprotein (VSV-G) overcomes the species barrier and is more resistant to mechanical
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