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GAPDH Polyclonal Antibody_货号:E-AB-40337_标签内参抗体
GAPDH Polyclonal Antibody
WB;IHC
Rabbit
Human;Mouse;Rat
12个月
E-AB-40337
科研级别
999
武汉伊莱瑞特生物科技股份有限公司
Unconjugated
Polyclonal
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
液体
IgG
500μL/300μL/120μL/60μL/30μL
规格: | 500μL | 产品价格: | ¥2835.0 |
---|---|---|---|
规格: | 300μL | 产品价格: | ¥1975.0 |
规格: | 120μL | 产品价格: | ¥875.0 |
规格: | 60μL | 产品价格: | ¥548.0 |
规格: | 30μL | 产品价格: | ¥295.0 |
Verified Samples |
Verified Samples in WB:A431,HepG2,Hela,HT29,A549,Jurkat,MCF-7,Rat heart,Mouse brain,Mouse heart,Rat brain
Verified Samples in IHC:Human bladder cancer,Human kidney,Mouse colon,Rat colon |
Dilution |
WB 1:3000-1:6000, IHC 1:300-1:500
|
一抗 |
1:5000
|
二抗 |
1:5000
|
封闭 |
2h
|
Clonality |
Polyclonal
|
Isotype |
IgG
|
Immunogen |
Recombinant Zebrafish Glyceraldehyde-3-phosphate dehydrogenase protein
|
Abbre |
GAPDH
|
Synonyms |
aging associated gene 9 protein; BARS-38; cb609; G3PDH; GAPD; KNC-NDS6; OCAS; p38 component抗体
|
Swissprot |
Q5XJ10
|
Calculated MW |
35 kDa
|
Observed MW |
35 kDa
|
Cellular Localization |
Cytoplasm, Cytoskeleton, Nucleus
|
Concentration |
0.66mg/mL
|
Storage |
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
|
Buffer |
PBS with 0.05% Proclin300, 50% glycerol, pH7.3.
|
Purification Method |
Antigen Affinity Purification
|
Research Areas |
Neuroscience; Isotype/Loading Controls; Signal Transduction; Cancer; Metabolism
|
Conjugation |
Unconjugated
|
Background |
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It was widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus post modification such as S-nitrosylation.
|
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