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- Elabscience
- E-AB-F1092H
- 湖北武汉
- 2025年07月15日
- FCM
- Rat
- Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
PE/Cyanine7 Anti-Mouse CD117/c-Kit Antibody流式抗体[2B8]_货号:E-AB-F1092H
- 抗体英文名：
PE/Cyanine7 Anti-Mouse CD117/c-Kit Antibody[2B8]_货号:E-AB-F1092H
- 应用范围：
FCM
- 宿主：
Rat
- 适应物种：
Mouse
- 保质期：
12个月
- 目录编号：
E-AB-F1092H
- 级别：
科研
- 库存：
999
- 供应商：
武汉伊莱瑞特生物科技股份有限公司
- 标记物：
PE/Cyanine7
- 克隆性：
Monoclonal
- 保存条件：
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
- 形态：
液体
- 亚型：
Rat IgG2b, κ
- 规格：
100Tests/50Tests/100Tests×2
| 规格： | 100Tests | 产品价格： | ￥1620.0 |
|---|---|---|---|
| 规格： | 50Tests | 产品价格： | ￥972.0 |
| 规格： | 100Tests×2 | 产品价格： | ￥2880.0 |
货号：E-AB-F1092H
| Background |
CD117 is a 145 kD immunoglobulin superfamily member also known as c-Kit and stem cell factor receptor (SCFR). It is a transmembrane tyrosine-kinase receptor that binds the c-Kit ligand (also known as steel factor, stem cell factor, and mast cell growth factor). CD117 is expressed on hematopoietic stem cells (including multipotent hematopoietic stem cells, progenitors committed to myeloid and/or erythroid lineages, and T and B cell precursors), mast cells, and acute myeloid leukemia (AML) cells. CD117 interaction with its ligand is critical for the development of hematopoietic stem cells.
|
| Alternate Names |
Mast/stem cell growth factor receptor Kit;Kit;SCFR;Proto-oncogene c-Kit;Tyrosine-protein kinase Kit;CD117;
|
| Swissprot |
P05532
|
| Gene ID |
16590
|
| Clone No |
2B8
|
| Host |
Rat
|
| Reactivity |
Mouse
|
| Application |
FCM
|
| Isotype |
Rat IgG2b, κ
|
| Isotype Control |
PE/Cyanine7 Rat IgG2b, κ Isotype Control[LTF-2]
|
| Conjugation |
PE/Cyanine7
|
| Conjugation Information |
PE/Cyanine7 is designed to be excited by the Blue (488 nm), Green (532 nm) and yellow-green (561 nm) lasers and detected using an optical filter centered near 775 nm (e.g., a 780/60 nm bandpass filter).
|
| Storage Buffer |
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.
|
| Storage |
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
|
| Expiration date |
12个月
|
| Shipping |
冰袋
|
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中。5. 用无菌 PBS 将上述锥形试管填满（至 50 mL)。介绍样品制备。6. 洗涤。在室温、低速制动条件下，以 300 X g 离心分离试管 10 分钟。7. 裂解红细胞（BD 产品目录号：555899，10 x 溶液。在蒸馏水中使用 1 x 溶液。室温孵育 15 分钟。）8. 将 1x106 PBMC 重悬于 20 μL 染色缓冲液中（PBS 含有 5% 正常人血清）。9. 加入抗体：CD117-BV421 (c-kit)、CD7-BV510、CD57- BV605、CD8-BV650
Isotype-specific immunostaining with mouse primary antibodies on mouse tissue
) Briefly, substitute your anti H+L pan-reactive secondary antibody with an anti-isotype heavy chain specific secondary. Be aware of possible odd crossreactivties (e.g. many goat anti mouse IgG 1 heavy chain do stain rat IgG 2a
Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
Fuchs, H., Gailus‐Durner, V., Adler, T., Aguilar‐Pimentel, J.A., Becker, L., Calzada‐Wack, J., Da Silva‐Buttkus, P., Neff, F., Götz, A., Hans, W., Hölter, S.M., Horsch, M., Kastenmüller, G., Kemter, E., Lengger, C., Maier, H., Matloka, M
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