- ￥1450
- 赛多利斯
- SartolabP20; PES-MF 0.2µm; sterile; 10p
- 2026年04月13日
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文献和实验Analysis of Proteins using Small Format 2D Gel Electrophoresis
twice with PBS-A and lysed in 0.2 ml of 2D lysis buffer (0.01M Tris-HCl, pH 7.4, 1mM EDTA, 8M urea, 0.05M dithiothreitol, 10% (v/v) glycerol, 5% (v/v) Nonidet P-40, 200µg/ml RNAse A, 6% (w/v) pH 3.5-10 carrier ampholytes ("Resolyte", Merck
Analysis of Proteins using Small Format 2D Gel Electrophoresis
-A and lysed in 0.2 ml of 2D lysis buffer (0.01M Tris-HCl, pH 7.4, 1mM EDTA, 8M urea, 0.05M dithiothreitol, 10% (v/v) glycerol, 5% (v/v) Nonidet P-40, 200µg/ml RNAse A, 6% (w/v) pH 3.5-10 carrier ampholytes ("Resolyte", Merck Ltd.)) for 5 minutes on ice
Preparation of Bacterial Proteins for Analysis by 2D-PAGE
to the bacteria under study the following buffers are used. 2D Lysis Buffer 0.01M Tris-HCl, pH7.4 1mM EDTA 8M Urea 0.05M DTT 10% (v/v) glycerol 5%(v/v) NP40 6% (w/v) ampholytes The buffer is made up using high purity MilliQ
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