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- 详细信息
- 技术资料
- 库存:
现货
- 供应商:
CELLIGENT
- 英文名:
Dialyzed Fetal Bovine Serum
| 产品名称 | 货号 | 规格 |
| 透析型胎牛血清 | CG1130A | 500ml |
| CG1130B | 50ml*10 |
Fetal Bovine Serum (FBS) is the most frequently used growth supplement for cell culture media. FBS Dialyzed is suitable for special studies, where small molecules may interfere. The content of disruptive molecules, like amino acids, hormones, and cytokines is significantly reduced.
Description
Fetal Bovine Serum (FBS), Dialyzed is suitable for special studies involving nutritional parameters and applications, where small molecules may interfere. Through the particular manufacturing process, the content of disruptive molecules, like amino acids, hormones, and cytokines is significantly reduced. Our Fetal Bovine Serum (FBS), Dialyzed is produced with a cut-off of 10 kDa. This makes it optimally suitable for proteomics and mitochondrial function analysis.
Fetal Bovine Serum (FBS), Dialyzed is suitable for studies involving nutritional parameters or incorporation of labeled material. These applications require the precise removal of certain constituents and whole serum cannot be used. The most common method for removal of these constituents is the dialysis of whole serum. Fetal Bovine Serum (FBS), Dialyzed is produced with a cut-off of 10 kDa.
Fetal bovine serum (FBS) or fetal calf serum (FCS) is widely used in eukaryotic cell culture, typically as a supplement for growth media. Fetal bovine serum contains nutritional and embryonic growth promoting factors that are essential for a successful cell culture. Further, fetal bovine serum contains various small molecules, such as, e.g., amino acids, lipids, sugars, and hormones.
Fetal bovine serum (FBS) is the blood component obtained after coagulation and removal of cellular components. Its unique and comprehensive mix makes FBS one of the most important supplements for supporting cell growth and proliferation for in vitro cell culture.
Our fetal bovine serum for cell culture supports most cell lines and undergoes strict quality controls.
【产品介绍】
常规细胞培养需要添加血清来维持细胞的增殖能力,尽管血清可以满足一般细胞培养需求,但在进行营养成分优化和标记特定成分进行研究的实验中,仍需要去除某些特定的成分,常用的去除方法有透析。透析是将血清循环通过中空纤维,并在血清中补充生理盐水以维持渗透压,移除分子量小于10,000MW的小分子,如氨基酸、葡萄糖、盐离子和未结合的蛋白等。
血清通常用作细胞生长的添加物,拥有多种功能和用途,如:富含大分子蛋白,低分子量的营养素,作为氨基酸、碳水化合物、维生素的额外来源;提高介质的缓冲能力;可结合或中和有毒成分;减少细胞氧化应激,减少细胞凋亡;提供促进生长的因子,有助细胞适应培养环境。
【适用范围】
可用于研究蛋白质组学、同位素标记、细胞信号传导和报告基因分析等。
【使用方法】
血清从冰冻区中取出后(切勿直接将血清从-20℃进入37℃解冻,这样因温度改变太大,容易造成蛋白质凝集而出现沉淀),置于2℃~8℃中使之融化,解冻过程中间歇地轻轻摇晃均匀,待全部溶解后再分装或直接使用。完全融解的血清从4℃拿出,按要求比例(5%-20%)加基础培养基配置成完全培养基,建议其置于4℃冰箱存放并于1-2周内用完。
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CELLiGENT Dialyzed/透析型胎牛血清 CG1130B
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