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- Biotium
- 美国
- 96067
- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20 °C
- 保质期：
1年
- 英文名：
CF® Dye Succinimidyl Ester CF®700 96067
- 库存：
99
- 供应商：
Biotium
- CAS号：
1
CF® Dye SE/TFP Esters are amine-reactive forms of Biotium’s bright and photostable CF® Dyes. The succinimidyl ester (SE) group, also known as N-hydroxysuccinimide (NHS) esters, on the dye molecule reacts with primary amines such as lysine residues of antibodies or proteins, amine-modified oligonucleotides and other amine-containing molecules to form a stable amide linkage. CF® Dye TFP (tetrafluorophenyl) esters are more stable alternatives to succinimidyl ester. CF® Dye TFP esters are available for CF®850 and CF®870, Biotium’s industry-leading near-infrared dyes with emission above 850 nm.
- Amine-reactive fluorescent dyes with high labeling efficiency
- Label antibodies, proteins, amine-modified oligonucleotides and other amine-containing molecules.
- Offered with Biotium’s bright and photostable CF® Dyes which provide superior signal-to-noise
- Available in 36 CF® Dye colors from blue to near-IR wavelengths
- Near-IR CF®850 and CF®870 TFP esters offer emission above 850 nm
Due to Biotium’s unique manufacturing and packaging process, our amine-reactive CF® Dyes are provided with the highest purity and more stable ester dye forms than other vendors. As a result, our CF® Dye SE/TFP products retain more reactivity and provide a higher labeling efficiency for users.
Superior CF® Dyes
CF® Dyes are Biotium’s line of next-generation fluorescent dyes that have improved brightness, photostability, and water solubility compared to Alexa Fluor®, DyLight®, and other fluorescent dyes. Learn more about CF® Dyes. For more information download the CF® Dye Brochure.
Also see our CF® Dye SE Protein Labeling Kits and CF® Dye Mix-n-Stain™Antibody Labeling Kits.
CF® Dye SE/TFP Ester
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文献和实验Labeling Tubulin with Tetramethylrhodamine Succinimidyl Ester
.Take a 10-20μl aliquot and dilute with 400μl buffer.Read OD at 556 nm and calculate dye concentration based on the molar extinction coefficient of 50,000.D/P should be around 0.75 per tubulin monomer.
LABELING MUSCLE ACTIN WITH CARBOXYFLUORESCEIN SUCCINIMIDYL ESTER
. 6.While vortexing,add 166 µl (8.31 mg)CFSE stock solution to 1 ml of buffer 2. 7.Mix dye solution immediately with actin solution,by gentle pipeting or stirring.Incubate at room temperature for 1 hr. 8.Centrifuge in a 50Ti rotor at 40,000 rpm,4℃ for 1 hr
偶联物，最大限度的提高了信噪比。图2. CF染料的超强特异性；由于AF染料制备过程中含有大量带负电的磺化基团，容易非特异性结合正电基团，请看AF790 Dye与CF790的条带特异性对比。3. 优异的标记效率生物偶联后的活性染料一般很容易水解，会在运输，操作及储存中带来诸多不便，最终致染料结合效率低下。像Alexa Fluor® dyes,DyLight? dyes and IRDyes®等严重的磺化作用有较强的吸湿性，恶化水解作用等问题。例如： AlexaFluor® 488 在应用时琥珀酰亚胺脂酯
