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- Biotium
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- 29004R-5ug
- 2025年07月10日
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- 保存条件：
4°C 避光
- 保质期：
6个月
- 英文名：
Annexin V CF555 Dye Conjugates, Azide-Free, Lyophilized 29004R-5ug
- 库存：
99
- 供应商：
Biotium
- CAS号：
1
Annexin V is a 35-36 kDa protein that has a high affinity for phosphatidylserine (PS). In normal viable cells, PS is located on the cytoplasmic surface of the cell membrane. In apoptotic cells, however, PS is translocated from the inner to the outer leaflet of the plasmic membrane, making itself available for Annexin V binding. Near-IR CF® Dye Annexin V does not contain azide or other potentially toxic materials, and thus is suitable for in vivo imaging.
- Detect apoptotic cells with fast, reliable detection of phosphatidylserine externalization
- No azide or other potentially toxic materials or preservatives, suitable for for in vivo imaging
- Choice of 7 near-IR fluorescent CF® dye colors
- Biotium’s CF® dyes are far more photostable and brighter than other similar near-IR dyes
- Can be used for fluorescence microscopy, live cell imaging, and flow cytometry
Superior CF® Dyes
CF® dyes are far more photostable and brighter than other similar near-IR dyes, such as Alexa Fluor® 700, Alexa Fluor® 750, Cy®7 and IRDye® 800. Learn more about Near-IR CF® Dyes in the Near-IR Dye Flyer. Learn more about CF® Dyes.
Detect Apoptosis with Annexin V
We also offer Annexin V Conjugates, Azide-Free, and Lyophilized in colors ranging from UV to far-red for live cell staining. Annexin V Conjugate solutions (with azide) also are available with a large selection of CF® Dyes, biotin, R-PE, APC, and other labels. Annexin V is also available with other probes in our Apoptotic and Necrotic Staining Kits. See our full selection of Cell Viability and Apoptosis Assays.
Annexin V Near IR CF® Dye Conjugates
| Conjugation | Ex/Em | Size | Catalog No. | Dye Features |
|---|---|---|---|---|
| CF®680 | 681/698 nm | 25 ug | 29007 | CF®680 Features |
| CF®680R | 680/701 nm | 25 ug | 29070 | CF®680R Features |
| CF®700 | 695/720 nm | 25 ug | 29082 | CF®700 Features |
| CF®750 | 755/777 nm | 25 ug | 29006 | CF®750 Features |
| CF®770 | 770/797 nm | 25 ug | 29046 | CF®770 Features |
| CF®790 | 784/806 nm | 25 ug | 29047 | CF®790 Features |
| CF®800 | 797/816 nm | 25 ug | 29078 | CF®800 Features |
CF is a registered trademark of Biotium, Inc. Alexa Fluor, Texas Red, and DyLight are registered trademarks of Thermo Fisher Scientific.
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文献和实验Carbohydrate‐Oligonucleotide Conjugates
of mannose and galactose oligonucleotide conjugates by bi‐click chemistry. J. Org. Chem. 74:1218‐1222. Rostovtsev, V.V., Green, L.G., Fokin, V.V., and Sharpless, K.B. 2002
Materials 0.05 to 1 mg free reducing glycan, lyophilized (e.g., Sigma‐Aldrich, V‐labs, Carbosynth) Milli
Labeling Tubulin and Quantifying Labeling Stoichiometry
. For dyes such as Cy5 and Cy3, use a 5-pack for labeling ~25 mg. For most dyes we label for 30'-40' at 37¡C. For C2CF-SNHS (caged fluorescein), we have found it best to add the dye in two steps (20' apart) and label for 60' at 37¡C. After adding the dye
