Applications Key: W=Western Blotting Reactivity Key: H=Human Mk=Monkey Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
RANTES (R40) Antibody detects endogenous levels of total human RANTES protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg40 of human RANTES. Antibodies were purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from differentiated THP-1 cells, untreated or treated overnight with LPS, using RANTES (R40) Antibody.
Western Blotting
Western blot analysis of HeLa cells, mock transfected or transfected with human RANTES construct, using RANTES (R40) Antibody.
Background
RANTES/CCL5 (regulated upon activation, T cell expressed and secreted) is a member of the "C-C" or β family of chemokines that induce inflammation and are associated with a number of inflammatory disorders (1,2). RANTES is produced and secreted mainly by CD8+ T cells, macrophages, and platelets, as well as epithelial cells, fibroblasts and some solid tumors (2-7). RANTES acts as a chemoattractant and has other regulatory functions on a number of cell types including monocytes, memory T cells, NK cells, eosinophils, basophils, dendritic cells, and mast cells (3, 7-9). Signaling by RANTES is mediated by several G-protein coupled receptors (GPCRs), including CCR1, CCR3, CCR4 and CCR5.