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- Cell Signaling Technology
- 4340
- USA
- 2025年09月07日
- F
- Rabbit
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Rabbit IgG Isotype Control (Alexa Fluor 488 Conjugate)__®
- 抗原：
F/P ratio of 2-6
- 应用范围：
F
- 宿主：
Rabbit
- 保质期：
详见说明书
- 级别：
详见MSDS文件
- 库存：
大量
- 供应商：
CST
- 标记物：
Alexa Fluor 488
- 是否单克隆：
0
- 保存条件：
-20°c
- 规格：
100 ul/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: F=Flow Cytometry
Reactivity Key:
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | Source |
|---|---|---|---|
| F | Endogenous | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | The purified antibody is not directed against any known antigen. It functions as an isotype control for rabbit antibodies. |
| Source / Purification | The antibody was isolated from naive rabbit sera and prepared by affinity chromotography. The antibody was conjugated to Alexa Fluor® 488 under optimal conditions with a F/P ratio of 2-6. Flow Cytometry
Flow cytometric analysis of Jurkat cells, using Rabbit IgG Isotype Control (Alexa Fluor® 488 Conjugate) (red) compared to Phospho-Akt (Ser473) (193H12) Rabbit mAb (Alexa Fluor® 488 Conjugate) #2336, untreated (green) or LY294002 and wortmannin treated (blue). Flow Cytometry
Flow cytometric analysis of Jurkat cells, using Rabbit IgG Isotype Control (Alexa Fluor® 488 Conjugate) (red) compared to Phospho-Stat3 (Tyr705) (D3A7) Rabbit mAb (Alexa Fluor® 488 Conjugate) #4323, untreated (blue) or IFN-α treated (green). |
| Description | Rabbit IgG was conjugated to Alexa Fluor® 488 fluorescent dye under optimal conditions and tested in-house for direct flow cytometric analysis of human and mouse cells. |
| Directions for Use | This control antibody must be diluted to the same concentration (not dilution) as the specific antibody used for analysis. Higher background fluorescence may result if excessive amounts of rabbit IgG isotype control are used. Do not use this antibody at a 1:50 dilution as recommended for other CST Alexa Fluor® conjugated antibodies. |
| Background | Isotype control antibodies are used to estimate the nonspecific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody. |
| Application References |
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
Alexa Fluor® is a registered trademark of Molecular Probes, Inc. For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验偶联物，最大限度的提高了信噪比。图2. CF染料的超强特异性；由于AF染料制备过程中含有大量带负电的磺化基团，容易非特异性结合正电基团，请看AF790 Dye与CF790的条带特异性对比。3. 优异的标记效率生物偶联后的活性染料一般很容易水解，会在运输，操作及储存中带来诸多不便，最终致染料结合效率低下。像Alexa Fluor® dyes,DyLight? dyes and IRDyes®等严重的磺化作用有较强的吸湿性，恶化水解作用等问题。例如： AlexaFluor® 488 在应用时琥珀酰亚胺脂酯
细胞仪检测。或者继续 E1 步 DNA 染色。 E. DNA 染色（可选） 1. 将细胞重悬在 0.5 ml DNA 染色液中（例如 DRAQ5® #4084）。 2. 室温至少孵育 5 分钟。 3. 流式细胞仪进行分析。 ☀ 推荐的二抗： 抗兔： Anti-Rabbit IgG（H+L）， F（ab'）2 Fragment（Alexa Fluor® 488 Conjugate）#4412 Anti-Rabbit IgG（H+L）， F（ab
on silver film. 2. Alexa488 Model Immunoassay Mouse and rabbit IgGs, buffer components and salts (such as bovine serum albimun, sucrose) were from Sigma-Aldrich. Bocking solution was 1% bovine serum albumin, 1% sucrose, 0.05% NaN3 , 0.05% Tween
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