Applications Key: W=Western Blotting Reactivity Key: H=Human M=Mouse R=Rat Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Phospho-MDM2 (Ser166) Antibody detects endogenous levels of MDM2 only when phosphorylated at Ser166.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser166 of human MDM2. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from COS cells, untransfected or transfected with Wild-type or mutant (S166S186A) MDM2, using Phospho-MDM2 (Ser166) Antibody.
Western Blotting
Western blot analysis of extracts from MCF-7 cells treated with IGF-1 for the indicated times, using Phospho-MDM2 (Ser166) Antibody.
Background
MDM2, a ubiquitin ligase for p53, plays a central role in regulation of the stability of p53 (1). Akt-mediated phosphorylation of MDM2 at Ser166 and Ser186 increases its interaction with p300, allowing MDM2-mediated ubiquitination and degradation of p53 (2-4). Phosphorylation of MDM2 also blocks its binding to p19ARF, increasing the degradation of p53 (3).