- 询价
- Cell Signaling Technology
- 2580
- USA
- 2025年12月16日
- W
- Rabbit
- R,H,M
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- 详细信息
- 技术资料
- 抗体英文名：
Phospho-IRS-1 (Ser332/336) Antibody
- 抗原：
synthetic phosphopeptide corresponding to residues surrounding Ser332/336 of mouse IRS-1 (equivalent to Ser337/341 of human IRS-1)
- 应用范围：
W
- 宿主：
Rabbit
- 供应商：
CST
- 库存：
大量
- 保质期：
详见说明书
- 级别：
详见MSDS文件
- 适应物种：
R,H,M
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting
Reactivity Key: H=Human M=Mouse R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | R (H) (M) | Transfected Only | 180 | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Phospho-IRS-1 (Ser332/336) Antibody detects transfected levels of IRS-1 when phosphorylated at Ser332/336. It also detects IRS-1 protein when singly phosphorylated at Ser332 or Ser336 of human IRS-1. This antibody does not cross-react with other related phosphoproteins. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser332/336 of mouse IRS-1 (equivalent to Ser337/341 of human IRS-1). Antibodies are purified by peptide affinity chromatography. Western Blotting
Western blot analysis of cell extracts from CHO cells overexpressing insulin receptor and IRS-1, untreated or treated with insulin, using Phospho-IRS-1 (Ser332/336) Antibody (upper and middle) or IRS-1 Antibody #2382 (lower). The middle blot was treated with calf intestinal phosphatase (CIP) before antibody probing. |
| Background | Insulin receptor substrate 1 (IRS-1) is one of the major substrates of the insulin receptor kinase (1). IRS-1 contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2-domain containing proteins that mediate the metabolic and growth-promoting functions of insulin (2-4). IRS-1 also contains over 30 potential serine/threonine phosphorylation sites. Ser307 of IRS-1 is phosphorylated by JNK (5) and IKK (6) while Ser789 is phosphorylated by SIK-2, a member of the AMPK family (7). The PKC and mTOR pathways mediate phosphorylation of IRS-1 at Ser612 and Ser636/639, respectively (8,9). Phosphorylation of IRS-1 at Ser1101 is mediated by PKCθ and results in an inhibition of insulin signaling in the cell, suggesting a potential mechanism for insulin resistance in some models of obesity (10). GSK-3-mediated IRS-1 serine phosphorylation leads to inhibition of insulin-stimulated IRS-1 signaling. Ser332 and Ser336 of IRS-1 are situated in a glycogen synthase kinase-3 (GSK-3) consensus motif (SXXXS), and it has been shown that Ser332 is the actual GSK-3 phosphorylation site while Ser336 provides a " priming" site necessary for GSK-3 action (11).
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| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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