- 询价
- Cell Signaling Technology
- 2841
- USA
- 2025年11月04日
- W
- Rabbit
- H,M,R,B
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Phospho-C/EBPalpha (Ser21) Antibody
- 抗原：
synthetic phosphopeptide corresponding to residues surrounding Ser21 of human C/EBPalpha
- 应用范围：
W
- 宿主：
Rabbit
- 供应商：
CST
- 适应物种：
H,M,R,B
- 级别：
详见MSDS文件
- 库存：
大量
- 保质期：
详见说明书
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting
Reactivity Key: H=Human M=Mouse R=Rat B=Bovine
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H M (R) (B) | Endogenous | 45 | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Phospho-C/EBPalpha (Ser21) Antibody detects endogenous levels of C/EBPalpha only when phosphorylated at serine 21. This antibody does not cross-react with other phosphorylated C/EBP isoforms. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser21 of human C/EBPalpha. Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting
Western blot analysis of extracts of COS cells untransfected (lane 1), or transfected with wild-type mouse C/EBPalpha (lane 2), S21A (lane 3), and S21D (lane 4) mutants, using Phospho-C/EBPalpha (Ser21) Antibody (upper) or C/EBPalpha Antibody (lower). (Provided by Dr. Hanna Radomska, Beth Israel Deaconess Medical Center, Boston, MA). Western Blotting
Western blot analysis of extracts from mouse adipocytes treated with insulin for the indicated times, using Phospho-C/EBPalpha (Ser21) Antibody. |
| Background | CCAAT/enhancer-binding proteins (C/EBPs) are a family of transcription factors that are critical for cellular differentiation, terminal function, and inflammatory response (1). Six members of the family have been characterized (C/EBPα, β, δ, γ, ε, and ζ) and are distributed in a variety of tissues (1). Translation from alternative start codons results in two isoforms of C/EBPα (p42 and p30), which are both strong transcriptional activators (2). It has been reported that insulin and insulin-like growth factor-I stimulate the dephosphorylation of C/EBPα, which may play a key role in insulin-induced repression of GLUT4 transcription (3). Phosphorylation of C/EBPα at Thr222, Thr226, and Ser230 by GSK-3 seems to be required for adipogenesis (4).
|
| Application References |
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验建议做复孔，每次测定应同时 做 标准曲线。 2. 洗涤过程 很关键。洗涤不充分将导致精确度误差及 OD 值错误地升高。 3. 板条开封后剩余板条要再封好，保持 板条干燥 。 4. 本试剂盒宜置 4 o C冰箱保存。 IL-21背景会比较高 ， 但不影响样品检测 。 5. 本试剂盒仅用于科研，不能用于临床诊断！
建议做复孔，每次测定应同时 做 标准曲线。 2. 洗涤过程 很关键。洗涤不充分将导致精确度误差及 OD 值错误地升高。 3. 板条开封后剩余板条要再封好，保持 板条干燥 。 4. 本试剂盒宜置 4 o C冰箱保存。 IL-21背景会比较高 ， 但不影响样品检测 。 5. 本试剂盒仅用于科研，不能用于临床诊断！
人成纤维生长因子-21(FGF-21)ELISA试剂盒 说明书
人成纤维生长因子-21 ( FGF-21 )ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 FGF-21 单抗包被于酶标板上，标准品和样品中的 FGF-21与单抗结合，加入生物素化的抗人 FGF-21 ，形成免疫复合物连接在板上，辣根过氧化物酶标记的 Streptavidin 与生物素结合，加入底物工作液显蓝
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