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Phospho-c-Jun (Ser63) II Antibody
synthetic phosphopeptide corresponding to residues around Ser63 of human c-Jun
W, IP, IF-IC, F
Rabbit
详见说明书
大量
H,M,R,Mk,Pg
详见MSDS文件
CST
2
-20°c
100 ul (10 western blots)/300 ul (30 western blots)/carrier free & custom formulation / quantity
规格: | 产品价格: | ¥请询价 | |
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规格: | 100 ul (10 western blots) | 产品价格: | ¥请询价 |
规格: | 300 ul (30 western blots) | 产品价格: | ¥请询价 |
规格: | carrier free & custom formulation / quantity | 产品价格: | ¥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey Pg=Pig
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Applications | Reactivity | Sensitivity | MW (kDa) | Source |
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W IP IF-IC F | H M R Mk Pg | Endogenous | 48 | Rabbit |
Protocols |
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Specificity / Sensitivity | Phospho-c-Jun (Ser63) II Antibody detects endogenous levels of c-Jun only when phosphorylated at Ser63. This antibody does not recognize the phosphorylated forms of JunD or JunB. |
Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser63 of human c-Jun. Antibodies are purified by protein A and peptide affinity chromatography. Western BlottingWestern blot analysis of extracts from anisomycin or UV-treated NIH/3T3 cells, using Phospho-c-Jun (Ser63) II Antibody. Flow CytometryFlow cytometric analysis of Jurkat cells, untreated (blue) or Anisomycin-treated (green), using Phospho-c-Jun (Ser63) II Antibody compared to a nonspecific negative control antibody (red). IF-ICConfocal immunofluorescent analysis of HeLa cells, anisomycin-treated (left) or untreated (right) using Phospho-c-Jun (Ser63) II Antibody (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye). |
Background | c-Jun is a member of the Jun Family containing c-Jun, JunB and JunD, and is a component of the transcription factor AP-1 (activator protein-1). AP-1 is composed of dimers of Fos, Jun and ATF family members and binds to and activates transcription at TRE/AP-1 elements (reviewed in 1). Extracellular signals including growth factors, chemokines and stress activate AP-1-dependent transcription. The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73 through SAPK/JNK (reviewed in 2). Knock-out studies in mice have shown that c-Jun is essential for embryogenesis (3), and subsequent studies have demonstrated roles for c-Jun in various tissues and developmental processes including axon regeneration (4), liver regeneration (5) and T cell development (6). AP-1 regulated genes exert diverse biological functions including cell proliferation, differentiation, and apoptosis, as well as transformation, invasion and metastasis, depending on cell type and context (7-9). Other target genes regulate survival as well as hypoxia and angiogenesis (8,10). c-Jun has emerged as a promising therapeutic target for cancer, vascular remodeling, acute inflammation, as well as rheumatoid arthritis (11,12).
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Application References |
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know! |
Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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