- 询价
- Cell Signaling Technology
- 4662
- USA
- 2025年09月05日
- W
- Rabbit
- H,M,Mk
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 询价记录
- 技术资料
- 抗体英文名：
p73 Antibody
- 抗原：
synthetic peptide corresponding to residues near the amino-terminus of human p73
- 应用范围：
W
- 宿主：
Rabbit
- 库存：
大量
- 适应物种：
H,M,Mk
- 级别：
详见MSDS文件
- 供应商：
CST
- 保质期：
详见说明书
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting
Reactivity Key: H=Human M=Mouse Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H (M) (Mk) | Transfected Only | 80 | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | p73 Antibody detects levels of p73 independent of phosphorylation and acetylation only when derived from a transfected DNA construct |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino-terminus of human p73. Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting
Western blot analysis of extracts from HT-1376 cells, untreated or pervanadate-treated (1 mM, 20 minutes), using Phospho-p73 (Tyr99) Antibody #4665 (upper), p73 Antibody (middle), or p63 (4A4) Mouse mAb #4894 antibody (lower). Western Blotting
Western blot analysis of extracts from HeLa cells untransfected (lane 1) or transfected with p73 DNA (lanes 2 and 3). Overexpression of p73 can be specifically blocked by co-transfection with p73 siRNA #6371 (lane 3). p73 was detected using p73 Antibody and p42 was detected using p42 MAPK Antibody #9108. The p73 Antibody confirms silencing of exogenous p73 expression and the p42 MAPK Antibody is used to control for loading and siRNA specificity. |
| Background | The p53 family member, p73, exists in multiple isoforms/splice variants and can induce apoptosis and cell cycle arrest in response to DNA damage via its activity as a transcription regulator (1-3). Upon DNA damage, p73 is phosphorylated at Tyr99 by c-Abl, causing translocation to the nuclear matrix (4). DNA damage-induced acetylation of p73 at Lys321 by the acetyltransferase p300 has also been reported to enhance transcription of genes including that of p53AIP1 (5). Another report, however, indicates that p300 does not acetylate full length p73 in vivo (6).
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
Because endogenous levels of p73 protein are low, it may be difficult to detect endogenous p73 by western blot using p73 Antibody #4662. For Research Use Only. Not For Use In Diagnostic Procedures. |
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
- 作者
- 内容
- 询问日期
技术资料暂无技术资料 索取技术资料
