MCE Seamless DNA Assembly Plus Kit 无缝克隆试剂盒为新一代重组克隆试剂盒,一次反应可完成单个至多个 DNA 片段的重组。其中经优化的 Mix 预混了重组酶和反应缓冲液,并添加了辅助因子,可显著提高克隆的重组效率和对杂质的耐受度,最快仅需 5 分钟即可完成单片段重组,且阳性率高达 95% 以上。
Description & Advantages:
Based on gene recombination, seamless cloning is a simple, efficient, and fast DNA cloning technology. A 15-20 bp sequence homologous to the end of the linearized vector is added to the 5´ end of the insert fragment primers. In the presence of enzymes, recombination can be easily achieved.
MCE Seamless DNA Assembly Plus Kit contains an optimized mix of recombinase, reaction buffer, and additional cofactors that significantly improve the cloning efficiency and tolerance to impurities. This product can complete multiple DNA fragments recombination and takes only 5 minutes for single fragment, and the positive rate is more than 95%.