MAP2 Antibody

4542:

Immunofluorescence , Western Blotting

MAP2 Antibody detects endogenous levels of all isoforms of MAP2 total protein. This antibody does not cross-react with tau.

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to carboxy-terminal residues of human MAP2. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western blot analysis of extracts from PC12 cells, untreated or nocodazole-treated, using MAP2 Antibody.

Western Blotting

Western blot analysis of extracts from COS cells, untransfected or MAP2C transfected, using MAP2 Antibody. (Human MAP2C construct is a generous gift from Dr. B. Shafit-Zagardo, Dept. of Pathology, Albert Einstein College of Medicine, Bronx, NY.)

IF-IC

Confocal microscopic images of PC-12 cells showing cytoplasmic stain with MAP2 Antibody (A, red) compared to an isotype control (B). Blue pseudocolor = DRAQ5^® #4084 (fluorescent DNA dye).

IF-F

Confocal immunofluorescent analysis of rat cerebellum, hippocampus and spinal cord (as indicated) using MAP2 Antibody (green). Blue pseudocolor = DRAQ5^® #4084 (fluorescent DNA dye).

Microtubule-associated protein 2 (MAP2) is a neuronal phosphoprotein that regulates the structure and stability of microtubules, neuronal morphogenesis, cytoskeleton dynamics and organelle trafficking in axons and dendrites (1). Multiple MAP2 isoforms are expressed in neurons, including high molecular weight MAP2A and MAP2B (280 and 270 kDa), and low molecular weight MAP2C and MAP2D (70 and 75 kDa). Phosphorylation of MAP2 modulates its association with the cytoskeleton and is developmentally regulated. Serine 136, threonine 1620 and threonine 1623 of MAP2 are phosphorylated by GSK3 and p44/42 MAP kinases (2,3). Phosphorylation at threonine 1620/1623 by GSK3 inhibits MAP2 association with microtubules and microtubule stability (3).

1. Sanchez, C. et al. (2000) Prog. Neurobiol. 61, 133-168.
2. Berling, B. et al. (1994) Eur. J. Cell Biol. 64, 120-130.
3. Sanchez, C. et al. (2000) Eur. J. Cell Biol. 79, 252-260.

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !

• 4541 Phospho-MAP2 (Ser136) Antibody
• 4544 Phospho-MAP2 (Thr1620/1623) Antibody
• 9101 Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody
• 9102 p44/42 MAPK (Erk1/2) Antibody
• 9106 Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (E10) Mouse mAb
• 9107 p44/42 MAPK (Erk1/2) (3A7) Mouse mAb
• 9331 Phospho-GSK-3α/β (Ser21/9) Antibody
• 9336 Phospho-GSK-3β (Ser9) Antibody
• 9337 Phospho-GSK-3α (Ser21) (46H12) Mouse mAb
• 7071 Phototope^® -HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide

For Research Use Only. Not For Use In Diagnostic Procedures.