IQGAP1 Antibody

2293:

Western Blotting

IQGAP1 Antibody detects endogenous levels of total IQGAP1 protein. The antibody may cross-react with a 35kDa protein of unknown origin.

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human IQGAP1.

Western Blotting

Western blot analysis of extracts from various cell types, using IQGAP1 Antibody.

IQGAPs are scaffolding proteins involved in mediating cytoskeletal function. They contain multiple protein interaction domains and bind to a growing number of molecules including actin, myosin light chain, calmodulin, E-cadherin, and β-catenin (reviewed in 1). Through their GAP-related domains, they bind the small GTPases Rac1 and cdc42. IQGAPs lack GAP activity, however, and regulate small GTPases by stabilizing their GTP-bound (active) forms (2,3). Studies have shown that the function and distribution of the IQGAP proteins widely vary. IQGAP1 is ubiquitously expressed and has been found to interact with APC (4) and the CLIP170 complex (5) in response to small GTPases, promoting cell polarization and migration. Studies have suggested that IQGAP1 could play a part in the invasiveness of some cancers (6-8). IQGAP2, which is about 60% identical to IQGAP1, is expressed primarily in liver (3), but lower levels have been detected in the prostate, kidney, thyroid, stomach, and testis (9,10). It has been shown that IQGAP2 displays tumor suppressor properties (11). Less is known about the function of IQGAP3, but this protein is present in the lung, brain, small intestine, and testis (9) and is only expressed in proliferating cells (12), suggesting a role in cell growth and division.

1. Briggs, M.W. and Sacks, D.B. (2003) EMBO Rep. 4, 571-574.
2. Ho, Y.D. et al. (1999) J. Biol. Chem. 274, 464-470.
3. Brill, S. et al. (1996) Mol. Cell Biol. 16, 4869-4878.
4. Watanabe, T. et al. (2004) Dev. Cell 7, 871-883.
5. Fukata, M. et al. (2002) Cell 109, 873-885.
6. Chew, C.S. et al. (2005) Am J Physiol Gastrointest Liver Physiol 288, G376-87.
7. Jin, S.H. et al. (2008) Int J Cancer 122, 1040-6.
8. Liu, Z. et al. (2010) Clin Cancer Res 16, 6009-18.
9. Wang, S. et al. (2007) J Cell Sci 120, 567-77.
10. Schmidt, V.A. et al. (2003) Blood 101, 3021-8.
11. Jin, S.H. et al. (2008) Int J Cancer 122, 1040-6.
12. Nojima, H. et al. (2008) Nat Cell Biol 10, 971-8.

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !

• 2462 Cdc42 Antibody
• 7071 Phototope^® -HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide

For Research Use Only. Not For Use In Diagnostic Procedures.