- 询价
- Cell Signaling Technology
- 9544
- USA
- 2025年11月01日
- W, IF-IC
- Rabbit
- M
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Cleaved PARP (Asp214) Antibody (Mouse Specific)
- 抗原：
synthetic peptide corresponding to carboxy-terminal residues surrounding Asp214 in mouse PARP
- 应用范围：
W, IF-IC
- 宿主：
Rabbit
- 保质期：
详见说明书
- 库存：
大量
- 适应物种：
M
- 供应商：
CST
- 级别：
详见MSDS文件
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
40 ul (4 western blots)/100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 40 ul (4 western blots) | 产品价格： | ￥请询价 |
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key: M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IF-IC | M | Endogenous | 89 | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Cleaved PARP (Asp214) Antibody (Mouse Specific) detects endogenous levels of the large fragment (89 kDa) of mouse PARP1 resulting from caspase cleavage. The antibody does not recognize full length PARP1 or other PARP isoforms. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to carboxy-terminal residues surrounding Asp214 in mouse PARP. Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting
Western blot analysis of NIH/3T3 cells, untreated or staurosporine-treated (1 µM, 3hrs), using Cleaved PARP (Asp214) Antibody (Mouse Specific). IF-IC
Confocal immunofluorescent analysis of 3T3-L1 cells, untreated (left) or staurosporine-treated (right), using Cleaved PARP (Asp214) Antibody (Mouse Specific) (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). |
| Background | PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress (1). This protein can be cleaved by many ICE-like caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa) (2,4). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6). (This product is sold under license from Promega Corp., U.S. Patent No. 6,350,452.)
|
| Application References |
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验monoclonal antibody production fusion
^6 cells/ml (IMDM + 10% FCS/FBS). Just before the fusion wash 3 times in IMDM (serum free!), 1200 rpm, 5min, 37ѓC. Count cells. sacrifice the mouse and dissect the spleen. tease the spleen in ice-cold IMDM (serum free!); pass
Assessment of Insulin Secretion in the Mouse
from the cleavage site ( 4 ). In the rat, two separate 110-amino acid preproinsulins are transcribed from two nonallelic preproinsulin genes, from which two forms of insulin and C-peptide are subsequently cleaved ( 1 ) ( see Fig
【求助】请问western blot做凋亡检测时，识别caspase-3酶原和激活的caspase-3的抗体是相同的吗？
lsdcfhyj 以及检测PARP的裂解前后蛋白水平用的抗体是否都是一样的？ edwardellen 有两者都识别的，也有专门识别剪切体不识别酶原的，用过cell signal的，两种都很好用。 PARP也有可以同时识别全长和剪切体的抗体。 magichunter 检测caspase-3和激活型的caspase-3分别需要两种不同的抗体，一般CST公司卖的分别包括：cleaved
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