AMPKα1 Antibody

2795:

Western Blotting

AMPKα1 Antibody detects endogenous levels of total AMPKα1. The antibody does not cross-react with AMPKα2.

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu519 near the carboxy terminus of human AMPKα1. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western blot analysis of extracts from HeLa, HT29 and COS cells, using AMPKα1 Antibody.

AMP-activated protein kinase (AMPK) is highly conserved from yeast to plants and animals and plays a key role in the regulation of energy homeostasis (1). AMPK is a heterotrimeric complex composed of a catalytic α subunit and regulatory β and γ subunits, each of which is encoded by two or three distinct genes (α1, 2; β1, 2; γ1, 2, 3) (2). The kinase is activated by an elevated AMP/ATP ratio due to cellular and environmental stress, such as heat shock, hypoxia, and ischemia (1). The tumor suppressor LKB1, in association with accessory proteins STRAD and MO25, phosphorylates AMPKα at Thr172 in the activation loop, and this phosphorylation is required for AMPK activation (3-5). AMPKα is also phosphorylated at Thr258 and Ser485 (for α1; Ser491 for α2). The upstream kinase and the biological significance of these phosphorylation events have yet to be elucidated (6). The β1 subunit is post-translationally modified by myristoylation and multi-site phosphorylation including Ser24/25, Ser96, Ser101, Ser108, and Ser182 (6,7). Phosphorylation at Ser108 of the β1 subunit seems to be required for the activation of AMPK enzyme, while phosphorylation at Ser24/25 and Ser182 affects AMPK localization (7). Several mutations in AMPKγ subunits have been identified, most of which are located in the putative AMP/ATP binding sites (CBS or Bateman domains). Mutations at these sites lead to reduction of AMPK activity and cause glycogen accumulation in heart or skeletal muscle (1,2). Accumulating evidence indicates that AMPK not only regulates the metabolism of fatty acids and glycogen, but also modulates protein synthesis and cell growth through EF2 and TSC2/mTOR pathways, as well as blood flow via eNOS/nNOS (1).

1. Hardie, D.G. (2004) J Cell Sci 117, 5479-87.
2. Carling, D. (2004) Trends Biochem Sci 29, 18-24.
3. Hawley, S.A. et al. (1996) J Biol Chem 271, 27879-87.
4. Lizcano, J.M. et al. (2004) EMBO J 23, 833-43.
5. Shaw, R.J. et al. (2004) Proc Natl Acad Sci USA 101, 3329-35.
6. Woods, A. et al. (2003) J Biol Chem 278, 28434-42.
7. Warden, S.M. et al. (2001) Biochem J 354, 275-83.

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• 4188 Phospho-AMPKα (Thr172) (D79.5E) Rabbit mAb
• 2535 Phospho-AMPKα (Thr172) (40H9) Rabbit mAb
• 2531 Phospho-AMPKα (Thr172) Antibody
• 2603 AMPKα (23A3) Rabbit mAb
• 2793 AMPKα (F6) Mouse mAb
• 2757 AMPKα2 Antibody
• 4182 AMPKβ1 Antibody
• 4150 AMPKβ1/2 (57C12) Rabbit mAb
• 4187 AMPKγ1 Antibody
• 2536 AMPKγ2 Antibody
• 2532 AMPKα Antibody
• 4148 AMPKβ2 Antibody
• 2550 AMPKγ3 Antibody
• 4184 Phospho-AMPKα1 (Ser485) Antibody
• 4185 Phospho-AMPKα1 (Ser485)/AMPKα2 (Ser491) Antibody
• 4181 Phospho-AMPKβ1 (Ser108) Antibody
• 4186 Phospho-AMPKβ1 (Ser182) Antibody
• 7071 Phototope^® -HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7727 Biotinylated Protein Ladder Detection Pack
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7003 20X LumiGLO^® Reagent and 20X Peroxide
• 9996 Oligomycin

For Research Use Only. Not For Use In Diagnostic Procedures.