MS1/19dd#7 rho-0 cells are a mtDNA-less derivative of MS1 (MILE SVEN 1), which are immortalized murine pancreatic islet endothelial cells. The MS1/19dd#7 cell line mtDNA depletion was achieved by ethidium bromide plus dideoxycytidine treatment in culture media for 19 days. Highlights: Useful as recipients of mtDNA in the production of cybridsScreened for the retention of mtDNAMS1 cell line is the only line known that can give rise to benign hemangiomas