含HRP-链霉亲和素的酪酰胺扩增试剂盒 and CF®640

This kit contains reagents for performing tyramide signal amplification (TSA) using streptavidin HRP conjugate and fluorescent CF® Dyes or biotin to increase immunofluorescence signal.

Features

• Increase sensitivity by up to 100-fold
• Use lower primary antibody concentrations
• Boost signal in challenging samples, like human FFPE tissues
• Choose from 6 bright & photostable CF® dyes or biotin

Kit Components:

• CF® dye or biotin-tyramide stock solution
• HRP Streptavidin
• BSA for blocking
• Tyramide Amplification Buffer Plus*
• 30% Hydrogen Peroxide*

Note: Endogenous biotin blocking reagents (not provided) may be required for streptavidin/biotin detection in cells or tissues.

Tyramide amplification, sometimes called Catalyzed Reporter Deposition (CARD), is an enzyme-mediated detection method that uses the catalytic activity of horseradish peroxidase (HRP) to generate high density labeling of a target protein or nucleic acid sequence in situ.  The tyramide amplification method has been reported to increase the sensitivity by up to 100-fold compared to conventional avidin-biotin detection. Multiple rounds of tyramide signal amplification can be performed for multiplex detection; to learn more, see our Tech Tip: Multicolor Fluorescence Imaging using Tyramide Amplification Kits.

Learn more about tyramide signal amplification.

These kits include your choice of 6 CF® dye tyramides or biotin tyramide. CF® dyes are Biotium’s line of next-generation fluorescent dyes, with advantages in brightness and photostability compared to other dyes. Learn more about CF® dyes.

Also see our Tyramide Amplification Kits with HRP Goat Anti-Mouse and Tyramide Amplification Kits with HRP Goat Anti-Rabbit.

Each kit contains reagents for staining 50-150 slides; the actual number of samples that can be stained will depend on staining volumes used. Tyramide Amplification Buffer, CF® Dye Tyramides, and other tyramide compounds are also available separately. See our full selection of tyramide products.

Tyramide Signal Amplification Kits

Tyramide Label	Ex/Em	Secondary conjugate	Catalog no.
CF®488A	490/515 nm	Goat anti-mouse HRP	33000
		Goat anti-rabbit HRP	33001
		Streptavidin HRP	33002
CF®543	541/560 nm	Goat anti-mouse HRP	33003
		Goat anti-rabbit HRP	33004
		Streptavidin HRP	33005
CF®568	562/583 nm	Goat anti-mouse HRP	33006
		Goat anti-rabbit HRP	33007
		Streptavidin HRP	33008
CF®594	593/614 nm	Goat anti-mouse HRP	33009
		Goat anti-rabbit HRP	33010
		Streptavidin HRP	33011
CF®640R	642/662 nm	Goat anti-mouse HRP	33012
		Goat anti-rabbit HRP	33013
		Streptavidin HRP	33014
CF®680R	680/701 nm	Goat anti-mouse HRP	33015
		Goat anti-rabbit HRP	33016
		Streptavidin HRP	33017
Biotin-XX	N/A	Goat anti-mouse HRP	33018
		Goat anti-rabbit HRP	33019
		Streptavidin HRP	33020

*Note: Older versions of this kit contain Ready-to-Use Tyramide Amplification Buffer (component 22027-25mL) without a separate vial of hydrogen peroxide. Download the product information sheet for detailed protocols. The SDS for the older version of the kit can be downloaded here: SDS-Tyramide-Amplification-Kit with 22027.