- 询价
- Cell Signaling Technology
- 2402
- USA
- 2025年08月27日
- W, IHC-P, IF-IC
- H,Mk
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 抗体英文名：
HSP27 (G31) Mouse mAb
- 抗原：
full length HSP27 protein
- 应用范围：
W, IHC-P, IF-IC
- 级别：
详见MSDS文件
- 库存：
大量
- 适应物种：
H,Mk
- 供应商：
CST
- 保质期：
详见说明书
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key: H=Human Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IHC-P IF-IC | H Mk | Endogenous | 27 | Mouse IgG1 |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | HSP27 (G31) Mouse mAb detects endogenous levels of total HSP27 protein. The antibody does not cross-react with other heat shock proteins. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with full length HSP27 protein. Western Blotting
Western blot analysis of extracts from SK-N-MC, HeLa and COS cells, using HSP27 (G31) Mouse mAb. Western Blotting
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-) or SignalSilence® HSP27 siRNA I (+) using HSP27 (G31) Mouse mAb and p44/42 MAPK (Erk1/2) (3A7) Mouse mAb #9107. The HSP27 (G31) Mouse mAb confirms silencing of HSP27 expression, while the p44/42 MAPK (Erk1/2) (3A7) Mouse mAb is used to control for loading and specificity of HSP27 siRNA. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using HSP27 (G31) Mouse mAb. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, untreated (left panels) or lambda phosphatase-treated (right panels), using Phospho-HSP27 (Ser82) Antibody #2401 (upper panels) or HSP27 (G31) Mouse mAb (lower panels). IF-IC
Confocal immunofluorescent analysis of A549 cells using HSP27 (G31) Mouse mAb (green). Red = Propidium iodide (fluorescent DNA dye). |
| Background | Heat shock protein (HSP) 27 is one of the small HSPs that are constitutively expressed at different levels in various cell types and tissues. Like other small HSPs, HSP27 is regulated at both the transcriptional and posttranslational levels (1). In response to stress, the HSP27 expression increases several-fold to confer cellular resistance to the adverse environmental change. HSP27 is phosphorylated at Ser15, Ser78, and Ser82 by MAPKAPK-2 as a result of the activation of the p38 MAP kinase pathway (2,3). Phosphorylation of HSP27 causes a change in its tertiary structure, which shifts from large homotypic multimers to dimers and monomers (4). It has been shown that phosphorylation and increased concentration of HSP27 modulates actin polymerization and reorganization (5,6).
|
| Application References |
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验(adjust pH to 7.8 with Binding buffer; red color) to the Protein A column.Mouse antibodies of the IgG1 subclass do not have a high affinity for protein A. Purification on protein A beads using standard conditions will yield approximately 1/10
Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers
T-Cell Activation Using mAb to CD3
One of the most common ways to assess T cell activation is to measure T cell proliferation upon in vitro stimulation of T cells via antigen or agonistic antibodies to TCR. This protocol is written as a starting point for examining in vitro proliferation of mouse
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