- 询价
- Cell Signaling Technology
- 2118
- USA
- 2025年07月24日
- western blot,免疫组化（IHC）,免疫荧光（IF）,流式细胞（Flow Cyt）
- 兔
- 人,小鼠,大鼠,驴,猪
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 抗体英文名：
GAPDH (14C10) Rabbit mAb
- 抗原：
/
- 应用范围：
western blot,免疫组化（IHC）,免疫荧光（IF）,流式细胞（Flow Cyt）
- 宿主：
兔
- 抗原来源：
/
- 保质期：
详见说明书
- 级别：
详见MSDS文件
- 适应物种：
人,小鼠,大鼠,驴,猪
- 供应商：
CST
- 库存：
大量
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/300 ul (30 western blots)/<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> /100 ul (10 western blots)/300 ul (30 western blots)/<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a>
| 规格： | 产品价格： | ￥面议 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | 300 ul (30 western blots) | 产品价格： | ￥请询价 |
| 规格： | <a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> | 产品价格： | ￥请询价 |
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | 300 ul (30 western blots) | 产品价格： | ￥请询价 |
| 规格： | <a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> | 产品价格： | ￥请询价 |
Product Pathways - Glucose Metabolism
GAPDH (14C10) Rabbit mAb #2118
Have you tried your application using our XP® monoclonal antibodies ? Try product: 5174
PhosphoSitePlus ® protein, site, and accession data: GAPDH
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IHC-P IF-IC F | H M R Mk (Pg) | Endogenous | 37 | Rabbit IgG |
Applications Key: W=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey Pg=Pig
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 2118:
- Flow , IHC / Paraffin , Immunofluorescence , Western Blotting
Specificity / Sensitivity
GAPDH (14C10) Rabbit mAb detects endogenous levels of total GAPDH protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide near the carboxy terminus of human GAPDH.
Western Blotting
Western blot analysis of extracts from various cell lines using GAPDH (14C10) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using GAPDH (14C10) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using GAPDH (14C10) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).
Flow Cytometry
Flow cytometric analysis of HeLa cells using GAPDH (14C10) Rabbit mAb antibody (blue) compared to a nonspecific negative control antibody (red).
IF-IC
Confocal immunofluorescent analysis of HeLa cells using GAPDH (14C10) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Background
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. Though differentially expressed from tissue to tissue (1), GAPDH is thought to be a constitutively expressed housekeeping protein. For this reason, GAPDH mRNA and protein levels are often measured as controls in experiments quantifying specific changes in expression of other targets. Recent work has elucidated roles for GAPDH in apoptosis (2), gene expression (3), and nuclear transport (4). GAPDH may also play a role in neurodegenerative pathologies such as Huntington and Alzheimer's diseases (4,5).
- Barber, R.D. et al. (2005) Physiol. Genomics 21, 389-95.
- Hara, M.R. and Snyder, S.H. (2006) Cell Mol. Neurobiol. 26, 527-38.
- Zheng, L. et al. (2003) Cell 114, 255-66.
- Bae, B.I. et al. (2006) Proc. Natl. Acad. Sci. USA 103, 3405-9.
- Wang, Q. et al. (2005) FASEB J. 19, 869-71.
Application References
- Bjorklund, N.L. et al. (2012) J Neurosci Methods 203, 146-51. Applications: Western Blotting
- Carlin, L.M. et al. (2011) Sci Signal 4, ra81. Applications: Western Blotting
- Siemens, N. et al. (2011) J Biol Chem 286, 21612-22. Applications: Western Blotting
- Wu, M. et al. (2010) Rejuvenation Res 13, 571-9. Applications: Western Blotting
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !
Companion Products
- 7071 Phototope® -HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 8112 SignalStain® Antibody Diluent
- 5425 Normal Goat Serum
- 9997 Tris Buffered Saline with Tween 20 (TBST-10X)
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
For Research Use Only. Not For Use In Diagnostic Procedures.
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文献和实验【信息】Rabbit anti-GAPDH(SF-PA005)（兔抗 GAPDH 多克隆 IgG 抗体）招代理
Rabbit anti-GAPDH(SF-PA005)（兔抗 GAPDH 多克隆 IgG 抗体） 背景 甘油醛 -3- 磷 酸脱氢酶（ Glyceraldehyde 3-phosphate dehydrogenase，GAPDH）是糖酵解（glycolysis）过程中的关键酶，分子量为37kDa 左右。它催化甘油醛-3-磷酸的氧化磷酸化反应。GAPDH 作为看家基因 在同种细胞 或者组织中一般是恒定、高水平表达的，常用作 Western Blot 实验的内参照. 来源 兔抗
7.8 or Binding Buffer.(6) BD tubing 14-170-12F (Fisher).(7) Polyethylene tubing Clay Adanes.(8) 18 Gauge needles.(9) Binding Buffer (BioRad): make 1L. 314g/L ddH2O and filter through 0.22 um, filter. pH=9.0.(10) Elution buffer (BioRad): make 500 ml. 11g/500 ml
Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers
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