The kit applies for detecting the level of anti-SARS-CoV-2 (2019-nCoV) Nucleocapsid antibodies in mouse serum.
Principle of the assay
The principle of the kit is indirect ELISA. SARS-CoV-2 (2019-nCoV) Nucleocapsid-His Recombiant Protein has been pre-coated onto well plate strips. The samples are added to the well, after incubation the wells are washed and a horseradish peroxidase conjugated Rabbit anti-Mouse IgG is added, producing an complex "antigen-antibody-labeled antibody". Following a wash, the TMB substrate solution is loaded and color develops in proportion to the amount of antibodies. The reaction is stopped by the addition of a stop solution and the intensity of the color can be measured at 450 nm.
Materials Provided
Components
1 Kit (96 Tests)
Microplate: Pre-coated with SARS-CoV-2 (2019-nCoV) Nucleocapsid-His Recombiant Protein