- 询价
- Biotium
- 美国
- 32003-T
- 2025年07月10日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 技术资料
- 保存条件：
-20°C
- 保质期：
1年
- 英文名：
Live-or-Dye 405/452 Fixable Viability Staining Kit, Trial Size (50 assays) 32003-T
- 库存：
99
- 供应商：
Biotium
- CAS号：
1
Live-or-Dye™ Fixable Viability Staining Kits allow discrimination between live and dead cells by flow cytometry or microscopy. Live/dead stains are useful probes to include when analyzing cell surface protein expression by flow cytometry, because they allow intracellular fluorescence signal from dead cells with permeable plasma membranes to be excluded from analysis.
Features
- Highly dead-cell specific
- Covalent labeling with reactive dyes
- Withstand fixation and permeabilization
- Wide selection of colors
- Dye options designed for spectral flow
- Sampler kits for convenience
Single Color Kit Components
- Live-or-Dye™ fixable dead cell dye
- Anhydrous DMSO
Sampler Kit Components
- 5 different Live-or-Dye™ fixable dead cell dyes
- Anhydrous DMSO
Biotium offers a wide selection of Live-or-Dye™ viability stains spanning the fluorescence spectrum, for maximal flexibility in multi-color analysis. Visit the Live-or-Dye™ Technology Page for more data and information.
How It Works
Live-or-Dye™ Fixable Viability Stains are cell membrane impermeant amine-reactive dyes. The dyes are able to enter into dead cells that have compromised membrane integrity and covalently label free amines on intracellular proteins. The dye labeling is extremely stable, allowing the cells to be fixed and permeabilized without loss of fluorescence or dye transfer between cells. The Live-or-Dye™ staining protocol has been optimized to maximize live/dead discrimination with minimal live cell staining, in order to prevent interference with immunostaining.
Biotium has a wide selection of Live-or-Dye™ viability stains spanning the fluorescence spectrum (see Table 1 below), for maximal flexibility in designing multi-color flow panels.
We also offer two sampler kits, each containing five different dye colors, for excitation from all of the popular flow cytometry laser lines (see Table 2 below). The Live-or-Dye™ Sampler Kit, Standard (32016) was designed for use on the most common flow cytometer laser and filter configurations, with dyes excitable by UV, Violet, Blue, Yellow-Green, and Red lasers. The Live-or-Dye™ Sampler Kit, Spectral (32017) was designed for use in spectral scanning flow cytometry. It contains dyes excitable by UV, Violet, Blue, and Red lasers, all of which have been validated on the Cytek® Aurora spectral cytometer, and chosen for their ability to fill the gaps in many spectral flow panels.
Live-or-Dye™ Fixable Viability Stains are dead cell specific in all cell types, including mammalian cells, bacteria and yeast. See our Cellular Stains Table for more information on how our dyes stain various organisms.
Table 1: Single-Color Live-or-Dye™ Kits
Cat. No. |
Viability Dye |
Compatible lasers (nm) |
Optimal detection channels |
Notes |
|---|---|---|---|---|
| 32018, 32018-T | Live-or-Dye™ 330/410 | 355, 375 | BUV395 | |
| 32002, 32002-T | Live-or-Dye™ 350/448 | 355, 375 | DAPI | |
| 32014,32014-T | Live-or-Dye™ 375/600 | 355, 375, 405 | Spectral scan, BUV615, BV605 | Developed for spectral cytometry |
| 32003, 32003-T | Live-or-Dye™ 405/452 | 405 | Pacific Blue®, BV421, BV450 | |
| 32009, 32009-T | Live-or-Dye™ 405/545 | 405 | AmCyan, BV510 | |
| 32004, 32004-T | Live-or-Dye™ 488/515 | 488 | FITC | Validated for microscopy |
| 32012, 32012-T | Live-or-Dye™ 510/550 | 488, 532 | Spectral scan | Developed for spectral cytometry |
| 32005, 32005-T | Live-or-Dye™ 568/583 | 488, 532, 561 | PE, PI | Validated for microscopy |
| 32006, 32006-T | Live-or-Dye™ 594/614 | 488, 532, 561 | PI, PE-CF®594, PE-Texas Red® | Validated for microscopy |
| 32015, 32015-T | Live-or-Dye™ 615/740 | 633-640 | Spectral scan, APC-Cy®7 | Developed for spectral cytometry |
| 32007, 32007-T | Live-or-Dye™ 640/662 | 633-640 | APC | Validated for microscopy |
| 32013, 32013-T | Live-or-Dye™ 665/685 | 633-640 | Spectral scan, AF700 | Developed for spectral cytometry |
| 32008, 32008-T | Live-or-Dye™ 750/777 | 633-640, 785 | APC-Cy®7 | The brightest dye for the APC-Cy®7 channel |
| 32011, 32011-T | Live-or-Dye™ 787/808 | 785, 808 | APC-Cy®7, IR800 |
Table 2: Live-or-Dye™ Sampler Kits
Kit Name |
Catalog No. |
Included dyes: Ex/Em (Cat No.) |
Application |
|---|---|---|---|
| Live-or-Dye™ Fixable Viability Sampler Kit, Standard | 32016 | • 350/488 (32002A) • 405/545 (32009A) • 488/515 (32004A) • 568/583 (32005A) • 640/662 (32007A) |
Designed for use on most standard flow cytometry laser and filter configurations |
| Live-or-Dye™ Fixable Viability Sampler Kit, Spectral | 32017 | • 350/448 (32002A) • 375/600 (32014A) • 510/550 (32012A) • 615/740 (32015A) • 665/685 (32013A) |
Designed for use in spectral flow cytometry, to fill in gaps between common fluorophores |
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
