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- 询价记录
- 文献和实验
- 技术资料
- 供应商:
科昊佳
- 库存:
现货
- 英文名:
Chicken Embryo Extract Powder/CEE
- 规格:
10ml
鸡胚提取物是从生长9-12天的鸡胚中提取的,用于组织培养中的生长因子补充成分。
鸡胚提取物是一种优质蛋白质,蛋白质含量高,氨基酸模式与人体的氨基酸模式一致,具有丰富的矿物元素和维生素,具有很高的营养价值。它的K、Fe、Se矿物元素和维生素A、D、B2等含量也很高。这使得它不但具有补充这些营养素的作用,而且,还可能有一些保健功能。已有实验证明,鸡胚提取物能有效地改善小鼠的硒营养不良状态,提高低硒小鼠的GPX活性,清除过氧化物产物MDA。有文献提出鸡胚提取物还具有调节血脂的功能。
该产品制备经无菌过滤(0.2μm),以10毫升冻干小瓶的形式提供。请注意,冻干产品无法保证无菌,重构后应通过无菌一次性过滤器重新过滤。
Description
The product is prepared in conformance with cGMP.
The proprietary process utilises 9-12 day eggs collected from registered flocks and found to be free from clinical signs of notifiable diseases.
It is processed as a 50% w/v extract in Dulbecco's Phosphate Buffered Saline. 100IU/ml Penicillin,100μg/ml Streptomycin and 2.5μg/ml Amphotericin are added as an additional safeguard.
Statistical samples representative of the batch are tested in agreement with cGLP to validate the product as being consistent with the designed quality standards of reliability and stability.
Typically used at a concentration of 0.1% to 2% in cell culture.
Directions for Use
1、Thaw the product overnight at 4°C and then bring to room temperature.
2、Use a 0.2um filtered ultra-purified water, and add to vials using a 10mL syringe with a hypodermic needle. Allow water to be draw in by vacuum.
3、Place samples on their sides and mix using an orbital shaker set at 150rpm at room temperature for 30 minutes.
4、To filter, use sterile gauze if necessary, then a 0.45um filter, then finally use a 0.2um filter.
| 产品名称 | Product | Cat no. | Pack Size |
| 胎牛血清 | Fetal Bovine Serum Standard | CG0430A | 500 ml |
| 胎牛血清盒装 | Fetal Bovine Serum Standard | CG0430B | 50mlx10 |
| 优级胎牛血清 | Fetal Bovine Serum Excellent | CG1126A | 500 ml |
| 优级胎牛血清盒装 | Fetal Bovine Serum Excellent | CG1126B | 50mlx10 |
| 胎牛血清(澳洲) | Fetal Bovine Serum Premium | CG0507A | 500ml |
| 胎牛血清(澳洲)盒装 | Fetal Bovine Serum Premium | CG0507B | 50mlx10 |
| 胎牛血清(新西兰) | Fetal Bovine Serum Premium II | CG1018A | 500ml |
| 胎牛血清(新西兰)盒装 | Fetal Bovine Serum Premium II | CG1018B | 50mlx10 |
| 猪血清 | Porcine Serum | CG1190 | 1000 ml |
| 马血清 | Equine Serum | CG1180A | 500ml |
| 马血清 | Equine Serum | CG1180B | 50mlx10 |
| 透析型胎牛血清 | Dialyzed FBS | CG1130A | 500ml |
| 透析型胎牛血清盒装 | Dialyzed FBS | CG1130B | 50mlx10 |
| 低IgG胎牛血清 | Low IgG FBS | CG1140A | 500ml |
| 低IgG胎牛血清盒装 | Low IgG FBS | CG1140B | 50mlx10 |
| 碳吸附胎牛血清 | Activated Charcoal Absorbed FBS | CG1120A | 500ml |
| 碳吸附胎牛血清盒装 | Activated Charcoal Absorbed FBS | CG1120B | 50mlx10 |
| 无外泌体胎牛血清 | Exosome Depleted FBS | CG1110A | 500ml |
| 无外泌体胎牛血清 | Exosome Depleted FBS | CG1110B | 50ml |
| 干细胞专用胎牛血清 | FBS, Embryonic Stem Cell Pre-tested (ES Cell FBS) | CG0713A | 500ml |
| 干细胞专用胎牛血清盒装 | FBS, Embryonic Stem Cell Pre-tested (ES Cell FBS) | CG0713B | 50mlx10 |
| 四环素阴性血清 | Tetracycline Negative FBS | CG1160A | 500 ml |
| 四环素阴性血清 | Tetracycline Negative FBS | CG1160B | 50mlx10 |
| 热灭火胎牛血清 | FBS, heat inactivated | CG1150A | 500 ml |
| 热灭火胎牛血清 | FBS, heat inactivated | CG1150B | 50mlx10 |
| 牛血清白蛋白 | BOVINE SERUM ALBUM | CG1310 | 100g |
| 牛血清白蛋白 | BOVINE SERUM ALBUM | CG1320 | 1kg |
| CEE鸡胚提取物 | Chicken Embryo Extract | CG1330 | 10ml |
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文献和实验Methylation analyis using restriction enzyme digestion
. Since in eukaryotic DNA (or actually in mammalian DNA) only cytosine in CG context can be methylated, the restriction enzymes with CG sequences within their restriction sites come in question. Two classical enzyme pairs are HpaII - MspI (CCGG) and SmaI - XmaI (CCCGGG
一、 什么是DNA甲基化在甲基转移酶的催化下,DNA的CG两个核苷酸的胞嘧啶被选择性地添加甲基,形成5-甲基胞嘧啶,这常见于基因的5'-CG-3'序列。大多数脊椎动物基因组DNA都有少量的甲基化胞嘧啶,主要集中在基因5'端的非编码区,并成簇存在。甲基化位点可随DNA的复制而遗传,?因为DNA复制后,甲基化酶可将新合成的未甲基化的位点进行甲基化。DNA 甲基化是表观遗传学的重要部分,同组蛋白修饰相互作用,通过改变染色质结构,调控基因表达。在哺乳类细胞或人体细胞中,DNA 甲基化与细胞的增殖、衰老
Analysis of methylation using bisulphite sequencing
from a cell line and then apply it to valuable samples. Before starting the experiment you have to develop primers for bisulphite converted DNA. You can generate a model of bisulphite treated DNA by substituting all cytosines which are not in CG context
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