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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 库存:
9999
- 英文名:
BSA (bovine serum albumin)
- 保质期:
5年
- 供应商:
科昊佳
- 保存条件:
-20℃
| 牛血清白蛋白BSA (bovine serum albumin) | ||
| 产品名称 | 货号 | 规格 |
| 牛血清白蛋白 | CG1310 | 100G |
| 牛血清白蛋白 | CG1320 | 1KG |
Bovine Serum Albumin (BSA) is a protein derived from bovine blood. It is used in fields like cell culture, molecular biology, protein biochemistry, and detection techniques.
Description
This Diagnostic Grade BSA Powder is designed to minimize analytes that cause background interference. Accordingly, this BSA can be used for sensitive immunoassays, including ELISAs (Enzyme-Linked Immunosorbent assay), immunoblots and immunohistochemistry, and as a stabilizing agent for protein reagents. Further applications include, e.g., for serum-free cell culture, as additive for hybridoma cell culture, protein stabilizer, and blocking agent.
牛血清白蛋白(BSA )是牛血浆中发现的主要蛋白质,在生命科学领域如细胞培养、体外诊断、人 和兽药、分子生物学、血清学和一般研究中都有应用。
•使用专有的两步纯化/热激工艺制造,可生产出质量一致的高质量BSA产品,非常适合需要高蛋 白纯度和低内毒素产品的各种应用。
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文献和实验Screen ES cells by Southern Blot
with “CG” Wash 3x10min. at 65℃ 4) Wrap membrane in Saran Wrap and exposure at -80℃ 4-7days “CG” Mix (for 100ml) 50ml 1M NaP, pH7.2 35ml 20% SDS 10ml 10% BSA 0.2ml 0.5M EDTA pH8.0 4.8ml H2 O“CG” Wash (for 2 liters) 80ml 1M NaP pH7.2 100ml 20% SDS
Screen ES cells by Southern Blot
a pencil to mark the wells. 7) Auto X-link membrane with Stratalinker, Ready to hybridize! 0.25M HCl: 10.4 ml conc HCl in 490 ml H20 0.4N NaOH: 24 ml 10N NaOH in 576 ml H20 Hybridization 1) Prehybridize membrane in “CG” Mix at 65 ‡C
PCR引物设计和优化(PCR PRIMER DESIGN AND REACTION OPTIMISATION)
, gelatin or BSA to 100ug/ml, and/or non-ionic detergents such as Tween-20 or Nonidet P-40 or Triton X-100 (0.05 - 0.10% v/v) (Innis and Gelfand, 1990)。 Modern formulations may differ considerably, however - they are also generally proprietary. PCR
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