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- Biotium
- 美国
- 92417
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20°C
- 保质期：
1年
- 英文名：
Mix-n-Stain Cyanine Dye Antibody Labeling Kits labeling with Cyanine 647,20-50ug labeling 92417
- 库存：
99
- 供应商：
Biotium
- CAS号：
1
Mix-n-Stain™ Cyanine Dye Antibody Labeling Kits allow you to label ≤5 ug up to 100 ug of your antibody in just 30 minutes, with minimal hands-on time and no purification.
Features
- Label 5-20 ug, 20-50 ug, or 50-100 ug Ab
- Less than 30 seconds of hands-on time
- 30 minutes total reaction time
- No purification, 100% recovery
- Compatible with BSA, gelatin, ascites
- Choose Cyanine 555 (identical to Cy®3) or Cyanine 647 (identical to Cy®5)
Kit components
- Ultrafiltration spin vial (for antibody concentration or buffer exchange, if needed)
- 10X Mix-n-Stain™ Reaction Buffer
- Lyophilized Reactive Dye
- Mix-n-Stain™ Storage Buffer
Unrivaled Convenience
Mix-n-Stain™ labeling tolerates common antibody buffer formulations. An ultrafiltration centrifuge vial is provided to quickly remove interfering substances like glycerol, or to concentrate your antibody. With a slight modification in protocol, antibodies can be labeled in the presence of BSA, gelatin, or ascites fluid. The presence of other proteins like BSA or gelatin in the labeling reaction has minimal effect on background fluorescence, because any labeled non-antibody proteins readily wash away during immunofluorescence staining.
Choose the Right Labeling Kit for Your Antibody
Mix-n-Stain™ Antibody Labeling Kits are very simple to use (see the workflow overview above). But before you begin, you must check that your antibody meets the compatibility requirements for labeling, and choose the right labeling protocol. Download the updated and expanded Product Protocol for a labeling check list to help you select the right kit size and labeling protocol for your antibody. Also see our Kit Compatibility and Protocol Selection Flowchart.
We offer Mix-n-Stain™ Kits for labeling antibodies, nanobodies, and small ligands with a wide selection of fluorescent dyes, enzymes, and other labels. See our full selection of Mix-n-Stain™ Antibody Labeling Kits below.
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文献和实验ORNL MICROARRAY HYBRIDIZATION PROTOCOLS
Buffer (Vial 6) Incubate at 65°C for 15-20 minutes Add probe mix to microarray and Hyb O/N at 55-65°C. Washes: 10'@55°C using 2XSSC, 0.2% SDS 10'@RT° using 2XSSC 10'@RT° using 0.2XSSC Image Arrays GENERAL
), provided that immunoreactivity is preserved and that a sufficiently high specific activity is achieved. The fluorescent antigen labeling method presented in this Chapter features the use of N―hydroxysuccinimido ester derivatives of a commercially available
