Mix-n-Stain™ Maxi CF®405S 抗体标记

Mix-n-Stain™ Maxi Antibody Labeling Kits allow you to rapidly label 1 mg of IgG with one of our next-generation CF® Dyes or other label.

• Rapid, simple, covalent antibody conjugation
• No purification required after labeling
• Reaction takes only 30 minutes, with minimal hands-on time
• Wide selection of our next-gen CF® Dyes and other dyes
• One labeling reaction of 1 mg IgG per kit

Kit Components

• Reaction Buffer
• Lyophilized Reactive Dye
• Quenching Buffer

Unrivaled Convenience

Mix-n-Stain™ Maxi Antibody Labeling Kits allow you to rapidly label 1 mg of IgG with one of our next-generation CF® Dyes or other label. Simply mix your antibody with the reaction buffer and optimally formulated dye provided, followed by a 30 minute incubation and addition of the provided stop buffer. Any remaining free dye or other label is no longer reactive at the end of the labeling, so the conjugate is ready for staining without further purification. Note the labeling protocol and buffer requirements for the Maxi kits differ from our original micro-scale Mix-n-Stain™ kits, see the Reaction Compatibility Chart below.

After the reaction, the antibody will be labeled with an average of 4-6 dye molecules per antibody molecule. Mix-n-Stain™ labeling is covalent, so labeled antibodies can be used for multiplex staining without transfer of dyes/labels between antibodies.

Superior CF® Dyes

These kits feature a choice of Biotium’s next-generation CF® Dyes, which were designed for advantages in brightness and photostability compared to Alexa Fluor®, DyLight®, and other fluorescent dyes. Learn more about CF® Dyes. We also offer kits for labeling with Cyanine 555 or Cyanine 647 (structurally identical to Cy®3 or Cy®5), or fluorescein/FITC. See Ordering Information below.

Choose the Right Kit for Your Application

These kits are optimized for a single labeling reaction with 1 mg of IgG. Mix-n-Stain™ kits are not designed to be used for multiple labeling reactions. For smaller-scale conjugation, see our original Mix‑n‑Stain™ CF® Dye Antibody Labeling Kits; original Mix-n-Stain™ kits also have greater tolerance for antibody buffer additives, and provide special protocols for labeling antibodies in buffer with BSA or in ascites fluid. The kits are not recommended for labeling IgM antibodies, which may denature under the reaction conditions.

Mix-n-Stain™ kits can be used to label other proteins, however, the degree of labeling (DOL, or number of dye molecules per antibody) may not be optimal, and the kits are not designed to allow for DOL measurement after labeling. For performing reactions to optimize DOL for your antibody or application, we recommend using our CF® Dye SE Protein Labeling Kits. The kits include everything you need to perform controlled conjugation reactions and post-labeling purification, as well as detailed instructions and correction factors for determining DOL for CF® Dyes.

We offer a variety of kits for labeling antibodies and other proteins:

• Mix-n-Stain™ Fluorescent Protein and Tandem Dye Antibody Labeling Kits
  with PE, APC, PerCP, and tandem dyes
• Mix-n-Stain™ Enzyme Antibody Labeling Kits
  with HRP, AP, and GOx
• CF® Dye SE Protein Labeling Kits
  for conjugation with post-labeling purification
• VivoBrite™ Rapid Antibody Labeling Kits for Small Animal In Vivo Imaging
  featuring best-in-class near-IR CF® Dyes
• Mix-n-Stain™ Small Ligand Labeling Kits
  generate your own fluorescent SNAP-tag®, CLIP-tag™ and HaloTag® ligands

Learn about all of our Mix-n-Stain™ and protein labeling options, or see our Frequently Asked Questions (FAQs) for more information.

Reaction Compatibility for Mix-n-Stain™ Maxi Antibody Labeling Kits, 1 mg Labeling

Component	Compatibility
Sodium azide	Compatible
Glycerol ≤ 10%:	Compatible
Glycerol > 10%:	Compatible after ultrafiltration*
Tris ≤20 mM	Compatible
Tris >20 mM	Compatible after ultrafiltration*
Glycine	Compatible after ultrafiltration*
BSA or gelatin	Not compatible; purify antibody**
Serum, cell culture supernatant, or ascites fluid	Not compatible; purify antibody**

* Ultrafiltration vials are sold separately (catalog no. 22004).
** Protocol/reagents for antibody purification not provided.

Mix-n-Stain™ Maxi Ordering Information

Conjugation	Ex/Em	Labeling size	Catalog No.	Dye Features
CF®350	347/448 nm	1 mg	92420	CF®350 Features
CF®405S	404/431 nm	1 mg	92421	CF®405S Features
CF®405M	408/452 nm	1 mg	92404	CF®405M Features
CF®405L	395/545 nm	1 mg	92454	CF®405L Features
CF®488A	490/515 nm	1 mg	92405	CF®488A Features
CF®555	555/565 nm	1 mg	92406	CF®555 Features
CF®568	562/583 nm	1 mg	92407	CF®568 Features
CF®594	593/614 nm	1 mg	92408	CF®594 Features
CF®633	630/650 nm	1 mg	92409	CF®633 Features
CF®647	650/665 nm	1 mg	92410	CF®647 Features
CF®680	681/698 nm	1 mg	92422	CF®680 Features
CF®750	755/777 nm	1 mg	92423	CF®750 Features
CF®770	770/797 nm	1 mg	92424	CF®770 Features
Fluorescein (FITC)	494/518 nm	1 mg	92411
Cyanine 555 (equivalent to Cy®3)	555/565 nm	1 mg	92415
Cyanine 647 (equivalent to Cy®5)	650/665 nm	1 mg	92419

Cy dye is a registered trademark of GE Healthcare.

 

Alexa Fluor and DyLight are registered trademarks of Thermo Fisher Scientific. Cy dye is a registered trademark of GE Healthcare; SNAP-tag and CLIP-tag are trademarks or registered trademarks of New England BioLabs; HaloTag is a registered trademark of Promega Corporation.