- 询价
- Biotium
- 美国
- 33015
- 2025年07月14日
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- 详细信息
- 技术资料
- 保存条件：
-20°C
- 保质期：
6个月
- 英文名：
Tyramide Amplification Kit with HRP Goat Anti-Mouse and CF680R Dye Tyramide 33015
- 库存：
99
- 供应商：
Biotium
- CAS号：
1
This kit contains reagents for performing tyramide signal amplification (TSA) using goat anti-mouse HRP secondary antibody and fluorescent CF® Dyes or biotin to increase immunofluorescence signal.
Features
- Increase sensitivity by up to 100-fold
- Use lower primary antibody concentrations
- Boost signal in challenging samples, like human FFPE tissues
- Choose from 6 bright & photostable CF® dyes or biotin
Kit Components:
- CF® dye or biotin-tyramide stock solution
- HRP Goat anti-mouse IgG (highly cross-adsorbed)
- BSA for blocking
- Tyramide Amplification Buffer Plus*
- 30% Hydrogen Peroxide*
Tyramide amplification, sometimes called Catalyzed Reporter Deposition (CARD), is an enzyme-mediated detection method that uses the catalytic activity of horseradish peroxidase (HRP) to generate high density labeling of a target protein or nucleic acid sequence in situ. The tyramide amplification method has been reported to increase the sensitivity by up to 100-fold compared to conventional avidin-biotin detection. Multiple rounds of tyramide signal amplification can be performed for multiplex detection; to learn more, see our Tech Tip: Multicolor Fluorescence Imaging using Tyramide Amplification Kits. Learn more about tyramide signal amplification.
The HRP goat anti-mouse antibody provided in the kits has been adsorbed against bovine, horse, human, rabbit, and swine serum to minimize cross-reactivity; the antibody is not cross-adsorbed against rat. The kits include your choice of 6 CF® dye tyramides or biotin tyramide. CF® dyes are Biotium’s line of next-generation fluorescent dyes, with advantages in brightness and photostability compared to other dyes. Learn more about CF® dyes.
Also see our Tyramide Amplification Kits with HRP Goat Anti-Rabbit and Tyramide Amplification Kits with HRP Streptavidin.
Each kit contains reagents for staining 50-150 slides; the actual number of samples that can be stained will depend on staining volumes used. Tyramide Amplification Buffer, CF® Dye Tyramides, and other tyramide compounds are also available separately. See our full selection of tyramide products.
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