Diphtheria Toxin

价格$620

品牌List Biological Labs
货号150
产地美国
更新日期2025年08月24日

北京孚博生物科技有限公司

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详细信息
询价记录
文献和实验
技术资料

库存：
20
英文名：
Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae
CAS号：
150
保质期：
1年
供应商：
北京孚博生物
保存条件：
2-8°C
规格：
1mg

List Labs 的核心是科学研究和对创新试剂的发现。在过去的四年中，我们提供包括毒素、肽、抗体和脂多糖在内的100多种细菌试剂产品的目录来为全球研究界提供服务。
List Labs 还为制药、生物技术、初创企业和政府组织提供各种服务，从技术转让到工艺开发，再到扩大到用于 1 期和 2 期临床供应的 cGMP 制造。针对须在BSL1到BSL3条件下研究的需氧菌、厌氧菌和孢子形成菌的方面，我们拥有专业的知识，并已被批准对根据前者制备的选择药剂和毒素的使用。我们还拥有GMP认证的能力，可以从我们的目录中或者通过用户定制产品来制造细菌产品和蛋白质。此外，List Labs在活性生物治疗产品 (LBP) 的 cGMP 制造方面拥有丰富的经验。

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以国内使用较多的百日咳毒素和白喉毒素为例介绍一下本公司的产品
产品细节图片1

1.百日咳毒素（Pertussis toxin）
百日咳毒素 (PTX) 是由百日咳博得特氏菌产生的，这种细菌是百日咳的罪魁祸首。百日咳毒素是一种多亚基蛋白，由六个非共价结合的亚基组成，分子量范围从大约9到28kDa。最大的亚基，也称为 A 原体，是一种响应ADP-核糖基转移酶活性的酶； A 原体单独将 ADP 核糖从 NAD+ 转移到 Gαi、Gαo 或 Gαt 类 G 蛋白的 α 亚基。五亚基结合复合物也称为 B 寡聚体，允许 PTX 进入大多数细胞，附着在受体蛋白（包括 TLR4 和糖蛋白 Ib）上的聚糖残基上。通过受体介导的内吞作用进入细胞后，PTX 通过内体途径和高尔基复合体逆行转运至内质网。 A 亚基从毒素中释放出来，并通过内质网膜易位，在那里毒素使靶膜结合的 G 蛋白失活。细胞生物学家使用 PTX 来消除 G 蛋白，从而确定 Gi/o 蛋白依赖性途径。由于毒素受体存在于几种哺乳动物细胞类型中，百日咳毒素影响大多数培养细胞。此外，百日咳毒素有助于创建自身免疫性疾病的动物模型。例如，百日咳毒素与髓鞘抗原一起施用于小鼠，以创建多发性硬化症、实验性自身免疫性脑脊髓炎 (EAE) 的小鼠模型。 List Labs 提供从百日咳博德特氏菌菌株 165 中分离出来的天然百日咳毒素。此外，List Labs 还生产一种无遗传活性的百日咳毒素，以及与百日咳博德特氏菌感染相关的几种毒力因子。百日咳毒素制剂是高度纯化的，包含所有五个亚基。百日咳制剂通过进行测试，确保将内毒素、腺苷酸环化酶和其他毒力因子的污染降至最低。并且每个活性测试批次都是在CHO细胞中进行的。
基本参数：
1. CAS NO：82248-93-9
2. 纯度：>95%
3. 生物活性：
a）CHO细胞实验—依照Hewlett et al.方法，产生阳性反应（CHO簇聚生长模式）所需PTX的最低浓度为0.03ng/ml；依批次不同而有变化。
b）腺苷酸环化酶实验：依照Wolff et al方法，在含1 μM钙调蛋白的体系中，PTX的腺苷酸环化酶活性是9picomoles/min/μg。依照批次不同会有差异。
List lab产品详情：

DESCRIPTION	FORMULATION	ABBREV.	PRODUCT #
Pertussis toxin from B. pertussis	Frozen in glycerol	PTX	179
Pertussis toxin from B. pertussis	Lyophilized in buffer	PTX	180
Pertussis toxin from B. pertussis	Lyophilized, salt-free	PTX	181
Pertussis toxin mutant	Frozen in glycerol	PTMUT	184
Adenylate Cyclase Toxin	Frozen in glycerol	ACT	188L
Filamentous hemagglutinin	Lyophilized in buffer	FHA	170
Fimbriae 2/3	Lyophilized in buffer	Fim	186
Pertactin, 69 kDa protein	Lyophilized in buffer	PRN	187

Product #179 Pertussis toxin from B. pertussis strain 165 in glycerol is provided frozen in a glycerol-containing Tris buffer. It will be shipped on ice.
Product #180 Pertussis toxin from B. pertussis strain 165, lyophilized in buffer is offered freeze-dried in phosphate buffer.
Product #181 Pertussis toxin from B. pertussis strain 165, lyophilized salt-free is freeze-dried in water and may be reconstituted with water or the buffer of choice.
Product #184 Pertussis toxin mutant (R9K, E129A) is provided lyophilized in phosphate buffer and an accompanying C of A describes the CHO cell toxicity.

Pertussis Toxin Mutant List Labs sells a genetically inactivated form of pertussis toxin, Pertussis Toxin Mutant (PT_MUT). This protein may be used as an antigen and as a carrier protein for other antigens. Pertussis mutant protein is isolated from the TY-178 strain of Bordetella bronchiseptica. Site-directed mutagenesis was used to replace amino acid residues in the enzyme active site. Although diminished, this mutant retains some of the activity of the native toxin.

Product #188L Adenylate cyclase toxin is an active enzyme, produced as a recombinant protein in E. coli. This product is liquid in urea buffer and specific thawing and dilution instructions are provided in an accompanying C of A

Adenylate cyclase toxin (ACT) is an important virulence factor secreted by Bordetella pertussis. Encoded by the Bordetella pertussis cyaA gene, adenylate cyclase toxin is a single polypeptide, with an apparent molecular weight of 220 kDa. The N-terminal of this protein contains an adenylate cyclase domain which binds to host cell calmodulin and catalyzes unregulated conversion of cellular ATP to cAMP. The C-terminal, receptor binding domain, contains numerous repeat in toxin (RTX) motifs and is responsible for binding to the cellular receptor on the surface of immune cells, translocation to the cell cytosol and creating cation-selective pores in the host cell membrane. Adenylate cyclase toxin both suppresses and modulates the host immune system contributing to pathogenesis of B. pertussis. This toxin is unusual in its ability to cross mammalian cell membranes and locate within the cytosol. As a cell biology tool, adenylate cyclase has been used to deliver immunogenic epitopes, targeting antigen presenting cells.

Product #170 Filamentous hemagglutinin (FHA)

The initial step in establishing a Bordetella pertussis infection is the attachment of the bacteria to the epithelial lining of the host respiratory tract. Two B. pertussis virulence factors are key in this process, filamentous hemagglutinin (FHA) and fimbriae (Fim). FHA, which is both surface-associated and secreted by B. pertussis, is a multifunctional protein that promotes the attachment of the bacteria through several binding domains. Because FHA is highly immunogenic, it is included in many of the acellular vaccines. Additionally, FHA acts through multiple pathways to modulate the host immune response; an example of this type of activity is induction in macrophages of the secretion of both pro-inflammatory and anti-inflammatory cytokines. FHA, a large, 220 kDa helical protein is produced by List Labs from the native Bordetella pertussis strain 165. FHA is isolated, provided lyophilized in Tris buffer and is tested for purity and hemagglutination activity.

Product #186 Fimbriae 2/3

Fimbriae are extracellular proteins which, like FHA, participate in the attachment of bacteria to substrates. They are long structures which serve to tether the bacteria to surfaces. Based on their recognition by specific antisera, there are two fimbriae serotypes present in B. pertussis, fimbriae 2 (Fim 2) and fimbriae 3 (Fim 3). Fim 2/3 are composed of subunits which dissociate in SDS, producing components with apparent molecular weights of 22,500 and 22,000 Da for Fim 2 and Fim 3, respectively. Fim 2/3 is isolated from cultures of a Pertactin negative mutant derived from the Bordetella pertussis Wellcome strain 28 expressing a mixture of fimbriae 2 and fimbriae 3. Fim is provided lyophilized in a Tris buffer and tested for purity, endotoxin and reactivity to antibodies. Results are provided in an accompanying C of A. Refer to the C of A for special instructions regarding product reconstitution.

Product #187 Pertactin (PRN)

Pertactin (PRN) is a 69 kDa surface-located protein produced by Bordetella pertussis. Due to its immunogenic and protective properties, it is included in acellular pertussis vaccines. The contribution of this protein to Bordetella pathogenesis is under investigation; however, it appears to play a role in overcoming neutrophil-mediated clearance of Bordetella during early stages of establishing an infection. Pertactin is one of the most polymorphic B. pertussis proteins, occurring in the population in more than 13 variations. Pertactin offered by List is isolated from native cultures of B. pertussis strain 165 and is provided lyophilized in HEPES buffer. Preparations are checked for purity and reactivity to antibodies.

2.白喉毒素（Diphtheria Toxin ）
作用机理：
白喉毒素 (DT) 由白喉棒状杆菌的某些菌株合成，并作为无活性的酶原分泌，由一条分子量约为 58 kDa 的多肽链组成。当酶原被切割成两个片段 A 和 B，通过二硫键连接，然后被硫醇还原，释放出 N 端 A 片段（分子量 21 kDa）时，DT 酶的活性就会表达出来。 C 端 B 片段（分子量 37 kDa）没有明显的酶活性，但它是毒性所必需的。有证据表明，B 片段负责识别毒素并将其与细胞表面受体结合。白喉毒素使用 NAD 作为底物催化真核氨酰基转移酶 II (EF-2) 的 ADP 核糖基化，这是其对真核生物的毒性的基础。白喉毒素受体 (DTR) 是一种在细胞表面表达的肝素结合表皮生长因子样生长因子前体 (HB-EGF)。在猴细胞中，另一种膜蛋白 CD-9 增加了白喉毒素对 HB-EGF 的亲和力，这表明两种蛋白质可能共同作为受体发挥作用。哺乳动物细胞系对白喉毒素的敏感性随受体的存在而变化。 Monkey Vero 细胞高度敏感，仓鼠细胞中等敏感，而没有受体的大鼠和小鼠细胞则具有抗性。注射 DT 对正常小鼠的影响相对较小，前者可用于选择性地和暂时性地消耗特定类型的免疫细胞群，这些细胞与猿或人类 DTR 基因相连。以这种方式使用 DT 可以使免疫系统正常发育，然后选择性消除特定细胞。
List lab产品详情

DESCRIPTION	FORMULATION	ABBREV.	PRODUCT #
TOXIN
Diphtheria Toxin, unnicked from Corynebacterium diphtheriae	Lyophilized in Buffer	DT	150
TOXOID
Diphtheria Toxoid, from Corynebacterium diphtheriae	Lyophilized in Buffer	DTd	151
CRM
Diphtheria Toxin CRM Mutant	Lyophilized in Buffer	CRM 197	149A

Product #150 Diphtheria toxin, unnicked, from C. diphtheriae, is provided lyophilized in Tris buffer. As toxicity may vary by lot, each laboratory should determine the optimum dosage for each particular application.
Product #149 Diphtheria toxin CRM Mutant is provided lyophilized in sodium phosphate buffer.
Product #151 Diphtheria toxoid is provided lyophilized in sodium phosphate buffer. Cytotoxicity testing results are described on a C of A that is included with every shipment.