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通用抗体Anti-HEL-Human IgG1(L234F, L235E, P331S mutation)Isotype negative antibody
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Hen egg Lysozyme
- 亚型:
Human IgG1-Kappa
- 形态:
liquid
- 保存条件:
This antibody can be stored at 2°C to 8°C for 2 weeks under sterile conditions; -20°C for 3 months under sterile conditions; -70°C for 36 months under sterile conditions. Avoid repeated freeze-thaw
- 克隆性:
单克隆
- 标记物:
Unconjugated
- 适应物种:
Human
- 保质期:
This antibody can be stored at 2℃to 8℃for 2 weeks under sterile conditions;-20℃for 3 months under sterile conditions;-70℃ for 65 months under sterile conditions. Avoid repeated freeze-thaw cycles.
- 抗原来源:
NA
- 目录编号:
B654901
- 级别:
Research Use only
- 库存:
999
- 供应商:
Biointron
- 宿主:
HEK293
- 应用范围:
Negative Control
- 浓度:
Lot Specific
- 靶点:
HEL
- 抗体英文名:
Anti-HEL-Human IgG1 L234F/L235E/P331S Isotype negative antibody
- 抗体名:
Human IgG1 L234F/L235E/P331S Isotype negative antibody
- 规格:
1mg
Used as a negative control in various in vivo and in vitro researches.
蛋白纯度:> 95% as determined by SDS-PAGE
蛋白内毒素:<1EU/mg, Determined by LAL gel clotting assay
保存条件:This antibody can be stored at 2°C to 8°C for 2 weeks under sterile conditions; -20°C for 3 months under sterile conditions; -70°C for 36 months under sterile conditions. Avoid repeated freeze-thaw
上海百英生物科技股份有限公司(Biointron)成立于2012年,公司通过了ISO9001:2015体系认证和两化融合体系贯标,是国家高新技术企业、国家专精特新科技小巨人企业。
百英生物拥有高通量重组抗体表达平台,AbDropTM单细胞抗体开发平台,利用微流控分选与鉴定技术,快速筛选候选分子。拥有噬菌体库抗体开发平台,全人源抗体库、各种属免疫库等丰富的建库与淘选经验。拥有羊驼纳米抗体开发平台,为双抗研发、细胞治疗提供支持。拥有ECACC的CHO-K1细胞株全球授权权利,可提供高表达稳定细胞株的开发,PD小试工艺优化以及宿主细胞授权。拥有抗体人源化、亲和力成熟等抗体工程改造平台,以及全面的药效评价平台。还拥有大量现货与活性验证的体内实验用药物通用抗体等产品。

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文献和实验The history of isotype selection for therapeutic antibodies clearly demonstrates that solid understanding of the biologic effects mediated by immunoglobulin (Ig) subclasses IgG1, IgG2, IgG3, and IgG4 and their variants is a prerequisite
Isotype-specific immunostaining with mouse primary antibodies on mouse tissue
) Briefly, substitute your anti H+L pan-reactive secondary antibody with an anti-isotype heavy chain specific secondary. Be aware of possible odd crossreactivties (e.g. many goat anti mouse IgG 1 heavy chain do stain rat IgG 2a
Immunohistochemistry: Fluorescence Protocol 2.0
by centrifugation (400 x g for 5 minutes). Incubate the cells for 30 minutes at room temperature with 50 µL of a biotinylated secondary antibody (either biotin-donkey anti-goat IgG Fab2 diluted 1:700 or biotin-goat anti-mouse IgG1 or IgG2A or IgG2B diluted






