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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名:
CF488A Rabbit anti-FLAG, 1mg/mL 20432
- 靶点:
FLAG tag
- 浓度:
1 mg/mL
- 应用范围:
Flow cytometry, IHC, IF (cells or tissue sections), Western blot
- 宿主:
Rabbit
- 保质期:
6个月
- 目录编号:
20432
- 库存:
99
- 供应商:
Biotium
- 标记物:
CF488A
- 克隆性:
多克隆
- 保存条件:
-20°C
- 形态:
Liquid: PBS/50% glycerol/2 mg/mL BSA/0.05% azide
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文献和实验the 3 hour incubation Dynabeads™ M-280 Streptaviden magnetic beads (7x107 beads, ~100µl/sample) are added to the respective samples (note 1);5.The resultant siRNA/Flag complexes are then eluted with magnetic bead binding, the solution can be discarded
and pre-elution bead equilibration. 4.Bind sample for 2 hours 5.Elute four times with 1mg/ml of flag.Expect ~50% of the TFC4 in the WCE to bind to the beads.Almost all of the TFC4 bound to the beads will elute in the first 3 elutions. Anion Exchange
Purification of TFIIIC from yeast
and pre-elution bead equilibration. 4.Bind sample for 2 hours 5.Elute four times with 1mg/ml of flag.Expect ~50% of the TFC4 in the WCE to bind to the beads.Almost all of the TFC4 bound to the beads will elute in the first 3 elutions. Anion Exchange
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