Cot1, active 10ug

Active Motif ChIP 实验专家技术经验分享：如何判断 ChIP 成功与否？

了，但具体还要依蛋白而定。比如 H3K4me3 的 ChIP，通常阳性区域比阴性区域的富集度要高 100 倍以上才可以认为做的不错，但对于很多与染色质结合不是很紧密的蛋白，比如一些转录因子，可能富集度有 10 倍差异就不错了。一般对于已有报道的蛋白的 ChIP，可以参考别人的报道来判断；而对于没有报道的蛋白 ChIP，一般能做出有 4～8 倍以上的差异，就可以初步认为成功了。具体富集度是怎么计算的呢？举个例子来说明：用作一个 ChIP 反应的起始样品叫做 Input，比如你用 20 ug 染色质去做一个

Antibody Conjugation Methods for Active Targeting of Liposomes

clearance are formidable obstacles. It has also been shown that antibodies become immunogenic when coupled to liposomes (10 ,11 ), although in similar experiments with ovalbumin we have demonstrated that immunogenicity can be suppressed by formulating

Measurements of Active TGF Generated by Culture Cells

8 ), and LAP is disulfide-bonded to LTBP (9 ). Four LTBPs (LTBP-1, 2, 3, and 4) have been described (10 –14 ). Mature TGF-β must be released from the complex to bind to its high-affinity receptor and elicit its biological functions (15 ). This process