HITES medium supplemented with 2% fetal bovine serum HITES medium with 2% fetal bovine serum is formulated at the ATCC as follows: Dulbecco's medium : Ham's F12, 50:50 mix (ATCC® 30-2006) Insulin 0.005 mg/ml Transferrin 0.01 mg/ml Sodium selenite 30 nM Hydrocortisone 10 nM β-estradiol 10 nM HEPES 10 mM L-glutamine (ATCC® 30-2214) 2 mM (in addition to that in the base medium) Fetal bovine serum (ATCC® 30-2020) 2%