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- 详细信息
- 文献和实验
- 技术资料
- 库存:
99999
- 英文名:
MeDIP Kit
- CAS号:
暂无
- 保质期:
1年
- 供应商:
广州伯信生物科技有限公司
- 保存条件:
4℃、-20℃
- 规格:
40T
DNA甲基化免疫共沉淀技术(Methylated DNA immunoprecipitation,MeDIP 或mDIP),是一个大范围的染色体或基因组纯化技术,在分子生物学中被用于富集DNA甲基化序列。 MeDIP是通过5-甲基胞嘧啶(5mC)抗体富集高甲基化的DNA片段,富集到发生甲基化的DNA片段,并采用下游核酸检测技术检测全基因组范围内的甲基化位点。
技术流程:

产品优势:
1. 敏感性高,待测DNA中5-甲基胞嘧啶分布极不均衡也可检测。
2. 适用于各种样本类型。
3. 转化效率高,在3小时内完成富含GC的DNA的完全转化。
4. 独特的保护剂最大限度地避免DNA降解,保障了后续的检测实验的需求。
5. 优化的缓冲液体系能最大限度的提高处理后的DNA回收效率。
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文献和实验- Jiang Y, Ma C, Hu Y, Yang Y, Ma C, Wu C, Liu L, Wen S, Moynagh PN, Wang B, Yang S. ECSIT Is a Critical Factor for Controlling Intestinal Homeostasis and Tumorigenesis through Regulating the Translation of YAP Protein. Adv Sci (Weinh). 2023 Sep;10(25):e2205180. IF=15.100.
- Deng Q, Li H, Yue X, Guo C, Sun Y, Ma C, Gao J, Wu Y, Du B, Yang J, Zhang C, Zhang W. Smooth muscle liver kinase B1 inhibits foam cell formation and atherosclerosis via direct phosphorylation and activation of SIRT6. Cell Death Dis. 2023 Aug 22;14(8):542. IF=9.000.
- Yang L, Chen Y, Liu N, Lu Y, Li X, Ma W, Gan W, Li D. 5mC and H3K9me3 of TRAF3IP2 promoter region accelerates the progression of translocation renal cell carcinoma. Biomark Res. 2022 Jul 27;10(1):54. IF=11.100.
- Wu Q, Yan Y, Shi S, Qi Q, Han J. DNMT3b-mediated SPAG6 promoter hypermethylation affects lung squamous cell carcinoma development through the JAK/STAT pathway. Am J Transl Res. 2022 Oct 15;14(10):6964-6977. IF=2.200.
Cytotoxic T Lymphocytes in SV40 Infections
specific for the large tumor antigen, or T antigen (T-ag) encoded by SV40. In addition, the T-ag in transformed or tumor cells also serves as the target for cytotoxic T lymphocytes (CTL) that are capable of controlling tumor development (2 ). CTL responses
Biology of p53 and SV40 Large T Association
p53 was discovered because of its capacity to bind to SV40 large T antigen (SVLT) in transformed cell lines (1 ,2 ). It is a phosphorylated, 393 amino acid protein, with five blocks of sequences that are highly conserved
Role of SV40 Small t in Cell Lysis, Transformation, and Signaling
Simian virus 40 small t antigen (st) is encoded on a differentially spliced transcript of the SV40 early region. It shares its aminoterminal 82 amino acids with the large T antigen and contains a unique cysteine-rich carboxyterminal half
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资料下载:
Bes5202 MeDIP 1 40T 说明书(For One Group).pdf 附 (下载 10 次)
Bes5202 MeDIP 2 40T 说明书(For Two Group).pdf 附 (下载 6 次)
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